ArticleJournal of clinical microbiology1995
Clinical evaluation of a Mycobacterium tuberculosis PCR assay.
Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 2 syntheses or guidelines pooled it, 125 citations in OpenAlex.
- In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.BMC microbiology · 2005Pooled it
- Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis.Journal of clinical microbiology · 2003Pooled it
- Trial
- Amplification of Mycobacterium tuberculosis from peripheral blood.Journal of clinical microbiology · 1995Trial
- Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR.Journal of clinical microbiology · 1995Trial
- Smartphone-based pathogen diagnosis in urinary sepsis patients.EBioMedicine · 2018Article
- RNomic identification and evaluation of npcTB_6715, a non-protein-coding RNA gene as a potential biomarker for the detection of Mycobacterium tuberculosis.Journal of cellular and molecular medicine · 2017Article
- Nationwide investigation of Shiga toxin-producing Escherichia coli among cattle in Japan revealed the risk factors and potentially virulent subgroups.Epidemiology and infection · 2017Article
- Legionella prevalence and risk of legionellosis in Japanese households.Epidemiology and infection · 2017Article
- Genes essential for the morphogenesis of the Shiga toxin 2-transducing phage from Escherichia coli O157:H7.Scientific reports · 2016Article
- Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments.BMC infectious diseases · 2016Article
- Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.Microbes and environments · 2015Article
- Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum.The American journal of tropical medicine and hygiene · 2014Article
- Polymerase chain reaction targeting insertion sequence for the diagnosis of extrapulmonary tuberculosis.The Indian journal of medical research · 2014Article
- Comparison of IS6110 and 'short fragment' devR (Rv3133c) gene targets with phenotypic methods for diagnosis of Mycobacterium tuberculosis.Medical journal, Armed Forces India · 2013Article
- Rapid diagnosis of Mycobacterium tuberculosis with Truenat MTB: a near-care approach.PloS one · 2013Article
- Multivariate analyses revealed distinctive features differentiating human and cattle isolates of Shiga toxin-producing Escherichia coli O157 in Japan.Journal of clinical microbiology · 2011Article
- Sarcoptes mite from collection to DNA extraction: the lost realm of the neglected parasite.Parasitology research · 2009Review
- Persistent association of Mycobacterium ulcerans with West African predaceous insects of the family belostomatidae.Applied and environmental microbiology · 2008Article
- Comparison of in-house PCR with conventional techniques and Cobas Amplicor M. tuberculosis kit for detection of Mycobacterium tuberculosis.Yonsei medical journal · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
On the basis of previously published PCR primer sequences, we have designed a sensitive system for detecting DNA of the Mycobacterium tuberculosis complex (MTB) in patient sputum samples which employs a fast and simplified sample preparation method appropriate for routine diagnostic testing. In order to evaluate the accuracy of the PCR assay, we performed a prospective study with 103 patients, comparing PCR results with culture results of samples obtained from a parallel culture assay as well as with subsequent culture results. Using two MTB-specific PCR primer systems, we found 48 of 49 tuberculosis (Tb) patients to be PCR positive (PCR sensitivity, 0.98). Sixteen of 54 presumably non-Tb patients showed amplifiable MTB DNA (specificity, 0.7). The study demonstrates that for diagnostic applications of MTB PCR two MTB-specific primer pairs should be used. MTB infection is extremely unlikely in cases of MTB PCR-negative samples: with our method for the exclusion of active Tb, the validity of one PCR assay seems to be equivalent to those of at least three culturing procedures. Positive PCR results do not necessarily reflect active MTB infection. It remains to be shown whether positive PCR results in Tb-negative patients mean false-positivity, an early laboratory finding which predicts a subsequent reactivation of a prior Tb infection, or whether asymptomatic patients may carry PCR-amplifiable MTB DNA without any clinical relevance. It is important to point out that the validity of PCR results in clinical studies depends on the use of contamination controls parallel to all PCR steps and the simplicity of the DNA extraction method as well as on the specificity of the PCR results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.