ArticleThe Journal of cell biology1995
The exocytotic fusion pore of small granules has a conductance similar to an ion channel.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 132 citations in OpenAlex.
- Fusion Pores as Regulators of Quantal Size and Cellular Physiology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Patch Amperometry and Intracellular Patch Electrochemistry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Exocytosis of large-diameter lysosomes mediates interferon γ-induced relocation of MHC class II molecules toward the surface of astrocytes.Cellular and molecular life sciences : CMLS · 2020Article
- Fusion Pore Expansion and Contraction during Catecholamine Release from Endocrine Cells.Biophysical journal · 2020Article
- Article
- Molecular mechanism of fusion pore formation driven by the neuronal SNARE complex.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- The function of TRP channels in neutrophil granulocytes.Pflugers Archiv : European journal of physiology · 2018Review
- Mechanics of post-fusion exocytotic vesicle.Physical biology · 2017Article
- Fusion pores and their control of neurotransmitter and hormone release.The Journal of general physiology · 2017Review
- Kiss-and-Run Is a Significant Contributor to Synaptic Exocytosis and Endocytosis in Photoreceptors.Frontiers in cellular neuroscience · 2017Article
- Hemifusion in Synaptic Vesicle Cycle.Frontiers in molecular neuroscience · 2017Review
- Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state.Cellular and molecular life sciences : CMLS · 2016Article
- A structural role for the synaptobrevin 2 transmembrane domain in dense-core vesicle fusion pores.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Local electrostatic interactions determine the diameter of fusion pores.Channels (Austin, Tex.) · 2015Article
- Methods for cell-attached capacitance measurements in mouse adrenal chromaffin cell.Journal of visualized experiments : JoVE · 2014Article
- cAMP-mediated stabilization of fusion pores in cultured rat pituitary lactotrophs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Comparison of unitary exocytic events in pituitary lactotrophs and in astrocytes: modeling the discrete open fusion-pore states.Frontiers in cellular neuroscience · 2013Article
- High resolution electrophysiological techniques for the study of calcium-activated exocytosis.Biochimica et biophysica acta · 2012Review
- Permeation of styryl dyes through nanometer-scale pores in membranes.Biochemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We measured capacitance changes in cell attached patches of human neutrophils using a high frequency lock-in method. With this technique the noise level is reduced to 0.025 fF such that capacitance steps of 0.1 fF are clearly detected corresponding to exo- and endocytosis of single 60 nm vesicles. It is thus possible to detect almost all known exocytotic and endocytotic processes including exocytosis of small neurotransmitter containing vesicles in most cell types as well as endocytosis of coated and uncoated pits. In neutrophils we demonstrate a stepwise capacitance decrease generated by 60-165 nm vesicles as expected for endocytosis of coated and non-coated pits. Following ionomycin stimulation a stepwise capacitance increase is observed consisting of 0.1-5 fF steps corresponding to the different granule types of human neutrophils from secretory vesicles to azurophil granules. The opening of individual fusion pores is resolved during exocytosis of 200 nm vesicles. The initial conductance has a mean value of 150 pS and can be as low as 35 pS which is similar to the conductance of many ion channels suggesting that the initial fusion pore is formed by a protein complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.