ArticleHistochemistry1994
Improved in situ beta-galactosidase staining for histological analysis of transgenic mice.
Article in Histochemistry, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Analysis of Dip2B Expression in Adult Mouse Tissues Using the LacZ Reporter Gene.Current issues in molecular biology · 2021Article
- Analysis of Gene Expression Using lacZ Reporter Mouse Lines.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Overview and assessment of the histochemical methods and reagents for the detection of β-galactosidase activity in transgenic animals.Anatomical science international · 2016Review
- Hedgehog-PKA signaling and gnrh3 regulate the development of zebrafish gnrh3 neurons.PloS one · 2014Article
- A fast and sensitive alternative for β-galactosidase detection in mouse embryos.Development (Cambridge, England) · 2012Article
- Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue.Histochemistry and cell biology · 2012Article
- Hyccin, the molecule mutated in the leukodystrophy hypomyelination and congenital cataract (HCC), is a neuronal protein.PloS one · 2012Article
- Characterization of a heat resistant beta-glucosidase as a new reporter in cells and mice.BMC biology · 2010Article
- Cell-type specific interaction of endothelin and the nitric oxide system: pattern of prepro-ET-1 expression in kidneys of L-NAME treated prepro-ET-1 promoter-lacZ-transgenic mice.The Journal of physiology · 2007Article
- Targeted deletion of the epididymal receptor HE6 results in fluid dysregulation and male infertility.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study describes a novel method for the histochemical demonstration of beta-galactosidase activity on tissue sections. We have replaced 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) with 5-bromo-indolyl-beta-o-galactopyranoside (Bluo-Gal) as a chromogenic substrate for the bacterial beta-galactosidase (lacZ). After beta-galactosidic cleavage, Bluo-Gal precipitates in form of fine birefringent crystals, whereas X-gal gives rise to an amorphous precipitate. Upon microscopic examination under polarized light, the crystals emit a strong signal consisting of yellow reflected light. This property of Bluo-Gal results in greatly enhanced sensitivity of the staining method for beta-galactosidase and allows for optimal morphological resolution. To exemplify the applications of this technique, the expression is demonstrated in transgenic mice of beta-galactosidase driven by a fragment of the human tissue-type plasminogen activator promoter.
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Registered trials
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