ArticleProceedings of the National Academy of Sciences of the United States of America1995
Long-term culture system for selective growth of human B-cell progenitors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 80 citations in OpenAlex.
- Analysis of acute lymphoblastic leukemia drug sensitivity by changes in impedance via stromal cell adherence.PloS one · 2021Article
- On-chip recapitulation of clinical bone marrow toxicities and patient-specific pathophysiology.Nature biomedical engineering · 2020Article
- Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow-derived progenitor cells.American journal of veterinary research · 2017Article
- Erk Negative Feedback Control Enables Pre-B Cell Transformation and Represents a Therapeutic Target in Acute Lymphoblastic Leukemia.Cancer cell · 2015Article
- Early B lymphocyte development: Similarities and differences in human and mouse.World journal of stem cells · 2014Review
- B cell biology: an overview.Current allergy and asthma reports · 2014Review
- Review
- Stromal cell-free conditions favorable for human B lymphopoiesis in culture.Journal of immunological methods · 2010Article
- The TLR1/2 agonist PAM(3)CSK(4) instructs commitment of human hematopoietic stem cells to a myeloid cell fate.Leukemia · 2009Article
- IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Ageing, autoimmunity and arthritis: senescence of the B cell compartment - implications for humoral immunity.Arthritis research & therapy · 2004Review
- Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation.The Journal of experimental medicine · 2001Article
- Id2 and Id3 inhibit development of CD34(+) stem cells into predendritic cell (pre-DC)2 but not into pre-DC1. Evidence for a lymphoid origin of pre-DC2.The Journal of experimental medicine · 2000Article
- Stromal cell-independent differentiation of human cord blood CD34+CD38- lymphohematopoietic progenitors toward B cell lineage.Leukemia · 2000Article
- Human fetal liver cells induce colonies in spleen of lethally irradiated mice.Journal of clinical immunology · 1997Article
- Interleukin 7 independent development of human B cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe a simple reproducible system for enrichment and long-term culture of human B-cell progenitors. Enriched CD34+ cord blood mononuclear cells are seeded onto a murine stromal cell line to establish a biphasic culture system. These cultures are characterized by transient growth of myeloid cells followed by outgrowth of cells highly enriched for early B-cell progenitors. Cultures consisting of > 90% early B-lineage cells [expressing CD10, CD19, CD38, and CD45 but lacking CD20, CD22, CD23, and surface IgM] are maintained for > 12 weeks without growth factor addition. Cells remain predominantly germ line at the immunoglobulin locus and express only low levels of cytoplasmic mu chain, terminal deoxynucleotidyltransferase, and recombination-activating gene 1 product. They are unresponsive to the pre-B-cell growth factors interleukin 7 or stem cell factor, or both, suggesting that growth support is provided by a cross-reactive murine stromal cell factor. Cultured B-cell progenitors are generated in large numbers ( > 10(8) cells from a typical cord blood specimen) suitable for use in biochemical analysis and gene-transfer studies. This system should be useful for study of normal and abnormal early human B-lymphopoiesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.