ArticleThe Journal of physiology1994
Three cation influx currents activated by purinergic receptor stimulation in rat megakaryocytes.
Article in The Journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Why do platelets express KPlatelets · 2021Review
- Effect of MgClInternational journal of molecular sciences · 2021Article
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Acetylsalicylic acid enhances purinergic receptor-mediated outward currents in rat megakaryocytes.American journal of physiology. Cell physiology · 2010Article
- Article
- Molecular and electrophysiological characterization of transient receptor potential ion channels in the primary murine megakaryocyte.The Journal of physiology · 2006Article
- Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte.The Journal of physiology · 2004Article
- Properties of the demarcation membrane system in living rat megakaryocytes.Biophysical journal · 2003Article
- A study of P2X1 receptor function in murine megakaryocytes and human platelets reveals synergy with P2Y receptors.British journal of pharmacology · 2002Article
- Depolarisation-evoked Ca2+ waves in the non-excitable rat megakaryocyte.The Journal of physiology · 2001Article
- Voltage-dependent Ca2+ release in rat megakaryocytes requires functional IP3 receptors.The Journal of physiology · 2001Article
- ADP is not an agonist at P2X(1) receptors: evidence for separate receptors stimulated by ATP and ADP on human platelets.British journal of pharmacology · 2000Article
- A novel role for membrane potential in the modulation of intracellular Ca2+ oscillations in rat megakaryocytes.The Journal of physiology · 2000Article
- Inhibition of store-operated Ca2+ entry by extracellular ATP in rat brown adipocytes.The Journal of physiology · 1999Article
- Depolarization-evoked Ca2+ release in a non-excitable cell, the rat megakaryocyte.The Journal of physiology · 1999Article
- ADP and inositol trisphosphate evoke oscillations of a monovalent cation conductance in rat megakaryocytes.The Journal of physiology · 1998Article
- Inhibition of inositol 1,4,5-trisphosphate-induced Ca2+ release by cAMP-dependent protein kinase in a living cell.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Extracellular nucleotides activate non-selective cation and Ca(2+)-dependent K+ channels in rat osteoclasts.The Journal of physiology · 1997Article
- ADP-induced rapid inward currents through Ca(2+)-permeable cation channels in mouse, rat and guinea-pig megakaryocytes: a patch-clamp study.The Journal of physiology · 1996Article
- Differentiation of the human monocytic cell line U937 results in an upregulation of the calcium release-activated current, ICRAC.The Journal of physiology · 1996Article
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2 authors.
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Abstract
1. Simultaneous patch clamp and fura-2 fluorescence measurements were used to study ATP-evoked membrane currents and intracellular [Ca2+] ([Ca2+]i) changes in rat megakaryocytes. 2. At negative potentials, under conditions that blocked K+ currents, 20 microM ATP activated a biphasic inward current and a concurrent biphasic increase in [Ca2+]i. The initial [Ca2+]i increase was due to Ca2+ influx whereas the delayed (1.70 +/- 0.13 s, mean +/- S.D.) increase was at least partly due to the release of internal Ca2+ stores. 3. The initial current was activated within 100 ms, inactivated within 1-4 s and was carried by both Na+ and Ca2+. 4. The delayed current was also transient and carried mainly by Na+ when Ca2+ buffering in the pipette was low. This Na+ conductance did not require an increase in [Ca2+]i for activation, but was triggered by inositol 1,4,5-trisphosphate (IP3), or a metabolite of IP3. 5. Buffering of [Ca2+]i changes with BAPTA revealed a third current activated by Ca2+ release from internal stores. This channel was selective for divalent cations with the permeability sequence Ca2+ >> Ba2+ > Mn2+, Mg2+. 6. Adenosine-5'-O-3-thiotriphosphate (ATP gamma S), like ATP, evoked all three influx currents, whereas ADP only stimulated Ca2+ release and the two currents associated with it. Increasing the external divalent cation concentration abolished the ATP-evoked Ca2+ release and delayed currents but not the initial transient current. 7. We conclude that rat megakaryocytes express two types of purinergic receptor. One type, activated by ATP, is closely coupled to a non-selective cation channel.(ABSTRACT TRUNCATED AT 250 WORDS)
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