ArticleHistochemistry1994
Neuronal and endothelial nitric oxide synthase immunoreactivity and NADPH-diaphorase staining in rat and human pancreas: influence of fixation.
Article in Histochemistry, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 71 citations in OpenAlex.
- Vagal mechanisms as neuromodulatory targets for the treatment of metabolic disease.Annals of the New York Academy of Sciences · 2019Review
- Targeting eNOS in pancreatic cancer.Cancer research · 2012Article
- Calcium-dependent release of NO from intracellular S-nitrosothiols.The EMBO journal · 2006Article
- Free radicals and the pancreatic acinar cells: role in physiology and pathology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2005Review
- Nitric oxide and the pancreas: morphological base and role in the control of the exocrine pancreatic secretion.Molecular and cellular biochemistry · 2001Review
- Central nervous system nitric oxide synthase activity regulates insulin secretion and insulin action.The Journal of clinical investigation · 1998Article
- Electrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells.The Journal of physiology · 1997Article
- Nitric Oxide Synthase in Human Parathyroid Glands and Parathyroid Adenomas.Endocrine pathology · 1996Article
- Neural regulation of the endocrine pancreas.International journal of pancreatology : official journal of the International Association of Pancreatology · 1995Review
- Histochemistry of nitric oxide synthase in the nervous system.The Histochemical journal · 1995Review
- L-NNA inhibits the histochemical NADPH-d reaction in rat spinal cord neurons.Histochemistry and cell biology · 1995Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we wished to clarify the distribution and co-localization of nitric oxide synthase and NA-DPH-diaphorase (NADPH-d) in nerve cells, nerve fibres and parenchymal cells in exocrine and endocrine pancreas, and to assess the influence of fixation on the staining pattern obtained. For this purpose, we applied nitric oxide synthase immunocytochemistry and NADPH-d histochemistry to rat and human pancreas under different fixation conditions. Antibodies to neuronal and endothelial nitric oxide synthase were similarly applied. We found complete co-localization of neuronal nitric oxide synthase and NADPH-d in ganglion cells, and in nerve fibres around acini, excretory ducts, blood vessels and in islets of Langerhans of rat and human pancreas. Immunoreactivity for endothelial nitric oxide synthase was co-localized with NADPH-d in endothelial cells. However, in NADPH-d reactive islet and ductal epithelial cells we could detect neither brain nor endothelial nitric oxide synthase immunoreactivity with any fixation protocol applied. There were marked differences in NADPH-d staining of both neurons and parenchymal cells under different fixation conditions. These results indicate the existence of different types of NADPH-d, which are associated or not associated with nitric oxide synthase(s), and which are differently influenced by various fixation procedures in rat and human pancreas.
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