ArticleHistochemistry1994
Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies.
Article in Histochemistry, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen.Virchows Archiv : an international journal of pathology · 2019Article
- Gross handling of pulmonary resection specimen: maintaining the 3-dimensional orientation.Journal of thoracic disease · 2019Review
- Pathologic conditions of hard tissue: role of osteoclasts in osteolytic lesion.Histochemistry and cell biology · 2018Review
- Article
- The role of histopathology in forensic practice: an overview.Forensic science, medicine, and pathology · 2014Review
- A comparative antibody analysis of pannexin1 expression in four rat brain regions reveals varying subcellular localizations.Frontiers in pharmacology · 2013Article
- Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride.Biotechnic & histochemistry : official publication of the Biological Stain Commission · 2012Article
- Standardised fluorescence in situ hybridisation in cytological and histological specimens.Virchows Archiv : an international journal of pathology · 2005Article
- IGF-1R, IGF-1 and IGF-2 expression as potential prognostic and predictive markers in colorectal-cancer.Virchows Archiv : an international journal of pathology · 2003Article
- Simultaneous medullary and papillary microcarcinoma of thyroid in a patient with secondary hyperparathyroidism.Endocrine pathology · 2002Article
- Seminal vesicle autoantigen, a novel phospholipid-binding protein secreted from luminal epithelium of mouse seminal vesicle, exhibits the ability to suppress mouse sperm motility.The Biochemical journal · 1999Article
- Concentration dependent and adverse effects in immunohistochemistry using the tyramine amplification technique.The Histochemical journal · 1999Article
- Classical Hodgkin's disease. Clinical impact of the immunophenotype.The American journal of pathology · 1997Article
- Tissue preparation for immunocytochemistry.Journal of clinical pathology · 1997Article
- Lymphocyte-predominant Hodgkin's disease. An immunohistochemical analysis of 208 reviewed Hodgkin's disease cases from the German Hodgkin Study Group.The American journal of pathology · 1997Article
- Antigen retrieval, signal amplification and intensification in immunohistochemistry.Histochemistry and cell biology · 1996Review
- Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS).Histochemistry and cell biology · 1996Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formaldehyde fixation of biopsy specimens for routine purposes has often been held responsible for the poor reproducibility of immunohistochemical studies. Recently, antigen retrieval (AGR) using microwave irradiation was described as a potential tool to enhance immunostaining. A comparison of conventional staining and staining after microwave heating was performed for 52 markers, using tissues fixed in formaldehyde for 24 h, 1 to 6 weeks and 3 years respectively, as well as consultant case material. After adequate duration of fixation (24 h), only a few markers (17%) showed better results after AGR, but this percentage was increased to 50% when tissues were fixed for longer periods. Maximal enhancement was obtained in the group of consultant cases (58% of tested markers demonstrated better staining results), in which the period of fixation and tissue processing was unknown. To achieve reliable enhancement with AGR, continuous heating (100 degrees C) should not be shorter than 20 min. In conclusion, AGR may become the most important tool to simplify and equalize immunohistochemical techniques, if critically evaluated.
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Registered trials
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