ArticleBritish journal of cancer1995
Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction.
Article in British journal of cancer, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 337 citations in OpenAlex.
- Circulating Tumour Cells as Prognostic Biomarkers in Colorectal Cancer: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Overexpression of dopa decarboxylase in peritoneal dissemination of gastric cancer and its potential as a novel marker for the detection of peritoneal micrometastases with real-time RT-PCR.British journal of cancer · 2004Trial
- Detection of circulating epithelial cells after surgery for benign breast disease.Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology · 2000Trial
- Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients.British journal of cancer · 1999Trial
- Molecular methods in cancer diagnostics: a short review.Annals of medicine · 2024Review
- From Raman to SESORRS: moving deeper into cancer detection and treatment monitoring.Chemical communications (Cambridge, England) · 2021Review
- Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma.Oncology letters · 2017Article
- Detection of circulating tumor cells with CK20 RT-PCR is an independent negative prognostic marker in colon cancer patients - a prospective study.BMC cancer · 2017Article
- The Potential for Circulating Tumor Cells in Pancreatic Cancer Management.Frontiers in physiology · 2017Review
- A combination of liver fluke infection and traditional northeastern Thai foods associated with cholangiocarcinoma development.Parasitology research · 2016Article
- Role of genetic detection in peritoneal washes with gastric carcinoma: The past, present and future.World journal of gastrointestinal oncology · 2016Review
- Assessment of Hyperthermic Intraperitoneal Chemotherapy to Eradicate Intraperitoneal Free Cancer Cells.Translational oncology · 2016Article
- Gastrointestinal tract metastasis from tubulolobular carcinoma of the breast: a case report and review of the literature.OncoTargets and therapy · 2014Article
- Article
- The clinical relevance of urine-based markers for diagnosis of bladder cancer.Medical oncology (Northwood, London, England) · 2011Article
- Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR.Journal of hematology & oncology · 2009Article
- Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells.Journal of hematology & oncology · 2008Article
- A hybrid machine learning-based method for classifying the Cushing's Syndrome with comorbid adrenocortical lesions.BMC genomics · 2008Article
- Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer.BMC cancer · 2006Article
- Molecular detection of circulating tumor cells in the peripheral blood of patients with colorectal cancer using RT-PCR: significance of the prediction of postoperative metastasis.World journal of surgery · 2006Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circulating cancer cells in the blood play a central role in the metastatic process. Their number can be very small and techniques for their detection need to be both sensitive and specific. Polymerase chain reaction (PCR) has been successfully used to detect small numbers of tumour cells in haematological cancer in which abnormalities in DNA are sufficiently consistent to make this possible. For most solid tumours this not yet feasible. However, we have found that reverse transcriptase (RT)-PRC for tissue-specific gene expression is a useful technique for identifying small numbers of circulating cells in melanoma and neuroblastoma patients. In this report we describe detection of colon carcinoma cells by RT-PCR using CK 20 mRNA as a marker. Unlike other cytokeratin genes examined (CK 8 and CK 19), CK 20 was not transcribed in normal haematopoietic cells. This suggests a role for RT-PCR in the detection of colon carcinoma metastasis in blood and bone marrow, using CK 20 as the target gene. Future analysis of clinical material will determine the clinical significance of this technique.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.