ArticleThe Journal of cell biology1995
Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Transgenic expression of the human growth hormone minigene promotes pancreatic β-cell proliferation.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Unique amino acid signatures that are evolutionarily conserved distinguish simple-type, epidermal and hair keratins.Journal of cell science · 2011Article
- Mutation of a major keratin phosphorylation site predisposes to hepatotoxic injury in transgenic mice.The Journal of cell biology · 1998Article
- Directed expression of keratin 16 to the progenitor basal cells of transgenic mouse skin delays skin maturation.The Journal of cell biology · 1998Article
- Ectopic expression of N-acetylglucosaminyltransferase III in transgenic hepatocytes disrupts apolipoprotein B secretion and induces aberrant cellular morphology with lipid storage.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Simple epithelium keratins are required for maintenance of hepatocyte integrity.The American journal of pathology · 1997Article
- Mutation of human keratin 18 in association with cryptogenic cirrhosis.The Journal of clinical investigation · 1997Article
- A transgenic mouse model with an inducible skin blistering disease phenotype.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Susceptibility to hepatotoxicity in transgenic mice that express a dominant-negative human keratin 18 mutant.The Journal of clinical investigation · 1996Article
- Gene targeting at the mouse cytokeratin 10 locus: severe skin fragility and changes of cytokeratin expression in the epidermis.The Journal of cell biology · 1996Article
- Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant.The Journal of cell biology · 1995Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To examine the role of keratin intermediate filament proteins in cell structure and function, transgenic mice were isolated that express a modified form of the human K14 keratin protein in liver hepatocytes. A modified K14 cDNA (K14.P) sequence was linked downstream of the mouse transthyretin (TTR) gene promoter and enhancer elements to achieve targeted expression in hepatocytes. Hepatocytes expressing high levels of the transgene were found to have abnormal keratin filament networks as detected by indirect immunofluorescence using an antibody specific for the transgene product. Light and electron microscopic level histological analysis of isolated liver tissue showed in many cases degenerative changes that included inflammatory infiltration, ballooning degeneration, an increase in fat containing vacuoles, and glycogen accumulation. These changes were most evident in older mice over four months of age. No indication of typical Mallory body structures were identified at either the light or electron microscopic level. To evaluate secretory function in transgenic livers, bile acid secretion rates were measured in isolated perfused liver and found to be approximately twofold lower than aged-matched controls. These findings indicate that expression of an abnormal keratin in liver epithelial cells in the in vivo setting can alter the structure and function of a tissue and suggest a role of the keratin network in cellular secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.