ArticleIn vitro cellular & developmental biology. Animal1994
Mouse pancreatic acinar/ductular tissue gives rise to epithelial cultures that are morphologically, biochemically, and functionally indistinguishable from interlobular duct cell cultures.
Article in In vitro cellular & developmental biology. Animal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- An injury-associated lobular microniche is associated with the classical tumor cell phenotype in pancreatic cancer.Nature communications · 2025Article
- Stress or injury induces cellular plasticity in salivary gland acinar cells.Cell and tissue research · 2020Article
- Pancreatic duct-like cell line derived from pig embryonic stem cells: expression of uroplakin genes in pig pancreatic tissue.In vitro cellular & developmental biology. Animal · 2019Article
- Development of a polarized pancreatic ductular cell epithelium for physiological studies.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
- Feeder-cell-independent culture of the pig embryonic stem cell-derived exocrine pancreatic cell line, PICM-31.In vitro cellular & developmental biology. Animal · 2018Article
- Metaplasia: tissue injury adaptation and a precursor to the dysplasia-cancer sequence.Nature reviews. Cancer · 2017Review
- β1 integrin is a crucial regulator of pancreatic β-cell expansion.Development (Cambridge, England) · 2013Article
- c-Jun/AP-1 is required for CCK-induced pancreatic acinar cell dedifferentiation and DNA synthesis in vitro.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Pancreatic ductal cells in development, regeneration, and neoplasia.The Journal of clinical investigation · 2011Review
- Reprogramming into pancreatic endocrine cells based on developmental cues.Molecular and cellular endocrinology · 2010Review
- Conservation of the TGFbeta/Labial homeobox signaling loop in endoderm-derived cells between Drosophila and mammals.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] · 2010Article
- Stimulation of cAMP signalling allows isolation of clonal pancreatic precursor cells from adult mouse pancreas.Diabetologia · 2006Article
- Insulin-producing cells derived from human pancreatic non-endocrine cell cultures reverse streptozotocin-induced hyperglycaemia in mice.Diabetologia · 2005Article
- Liver repopulation and correction of metabolic liver disease by transplanted adult mouse pancreatic cells.The American journal of pathology · 2001Article
- In vitro cultivation of human islets from expanded ductal tissue.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Hepatocyte growth factor/scatter factor induces a variety of tissue-specific morphogenic programs in epithelial cells.The Journal of cell biology · 1995Article
- A mouse model for the delta F508 allele of cystic fibrosis.The Journal of clinical investigation · 1995Article
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Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Most of the pancreatic exocrine epithelium consists of acinar and intralobular duct (ductular) cells, with the balance consisting of interlobular and main duct cells. Fragments of mouse acinar/ductular epithelium can be isolated by partial digestion with collagenase and purified by Ficoll density gradient centrifugation. We investigated whether previously developed culture conditions used for duct epithelium would result in the selective survival and proliferation of ductular cells from the acinar/ductular fragments. The fragments were cultured on nitrocellulose filters coated with extracellular matrix. After 2 to 4 wk the filters were covered with proliferating cells resembling parallel cultures of duct epithelium by the following criteria: protein/DNA ratio, light and electron microscopic appearance, the presence of duct markers (carbonic anhydrase [CA] activity, CA II mRNA, the cystic fibrosis transmembrane conductance regulator), the near absence of acinar cell markers (amylase and chymotrypsin), a similar polypeptide profile after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the presence of spontaneous and secretin-stimulated electrogenic ion transport. Both duct and ductular epithelia formed fluid-filled cysts in collagen gels and both could be subcultured. We conclude that acinar/ductular tissue gives rise to ductular cells in culture by some combination of acinar cell death and/or transdifferentiation to a ductular phenotype, accompanied by proliferation of these cells and preexisting ductular cells. These cultures may be used to investigate the properties of this part of the pancreatic duct system, from which most of the pancreatic juice water and electrolytes probably originates.
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Registered trials
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