Evidence map›Paper›PMID 7529602›Full record

ArticleJournal of assisted reproduction and genetics1994

Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis.

C M Strom, S Rechitsky, G Wolf, Y Verlinsky

Abstract read
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Article in Journal of assisted reproduction and genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

C M StromReproductive Genetics Institute, Illinois Masonic Medical Center, Chicago 60657.
S Rechitsky
G Wolf
Y Verlinsky

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWe investigated the reliability of polymerase chain reaction (PCR) genotype analyses performed on single cells for the purposes of preimplantation genetic analysis.

methodsWe performed blind analysis of 130 single skin fibroblasts heterozygous for the delta-F508 mutation in the cystic fibrosis transmembrane regulator (CFTR) gene and 73 single skin fibroblasts from an individual heterozygous for the XbaI polymorphic site of the Factor VIII gene.

resultsAmplification was successful for 116 cells and 52 cells respectively and in all but one case (a CFTR analysis) both alleles were amplified. The incidence of diagnostic error was 1 out of 203 analyses or 0.0043. We conclude that PCR is a reliable method for determining the genotype of single cells for the purposes of preimplantation genetic analysis.

Indexed as

BlastocystPolymerase Chain ReactionPrenatal DiagnosisAllelesAmniotic FluidBase SequenceCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDeoxyribonucleases, Type II Site-SpecificDiagnostic ErrorsDNADNA PrimersFactor VIIIFetal DiseasesFibroblastsGenetic Carrier ScreeningCFTR protein, humanCystic Fibrosis Transmembrane Conductance RegulatorDeoxyribonucleases, Type II Site-SpecificDNADNA Primersendodeoxyribonuclease XBAIFactor VIIIMembrane Proteins

Identifiers

PMID7529602

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.