ArticleBiophysical journal1994
Estimation of kinetic rate constants from multi-channel recordings by a direct fit of the time series.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Similarity of the ion occupations of KChannels (Austin, Tex.) · 2026Article
- Using Bayesian priors to overcome non-identifiablility issues in Hidden Markov models.bioRxiv : the preprint server for biology · 2025Article
- Binding kinetics of quaternary ammonium ions in Kcv potassium channels.Channels (Austin, Tex.) · 2024Article
- A deep learning approach to real-time Markov modeling of ion channel gating.Communications chemistry · 2024Article
- 2D-dwell-time analysis with simulations of ion-channel gating using high-performance computing.Biophysical journal · 2023Article
- Asymmetric Interplay Between KFrontiers in physiology · 2021Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter.FEBS letters · 2017Article
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- Saturation and microsecond gating of current indicate depletion-induced instability of the MaxiK selectivity filter.The Journal of general physiology · 2007Article
- Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.The Journal of membrane biology · 2006Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
- A subsequent fit of time series and amplitude histogram of patch-clamp records reveals rate constants up to 1 per microsecond.The Journal of membrane biology · 2005Article
- Distributions-per-level: a means of testing level detectors and models of patch-clamp data.The Journal of membrane biology · 2004Article
- The effects of non-identifiability on testing for detailed balance in aggregated Markov models for ion-channel gating.Biophysical journal · 2000Article
- The anomalous mole fraction effect in Chara: gating at the edge of temporal resolution.Biophysical journal · 2000Article
- Estimating single-channel kinetic parameters from idealized patch-clamp data containing missed events.Biophysical journal · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maximum-likelihood technique for the direct estimation of rate constants from the measured patch clamp current is extended to the analysis of multi-channel recordings, including channels with subconductance levels. The algorithm utilizes a simplified approach for the calculation of the matrix exponentials of the probability matrix from the rate constants of the Markov model of the involved channel(s) by making use of the Kronecker sum and product. The extension to multi-channel analysis is tested by the application to simulated data. For these tests, three different channel models were selected: a two-state model, a three-state model with two open states of different conductance, and a three-state model with two closed states. For the simulations, time series of these models were calculated from the related first-order, finite-state, continuous-time Markov processes. Blue background noise was added, and the signals were filtered by a digital filter similar to the anti-aliasing low-pass. The tests showed that the fit algorithm revealed good estimates of the original rate constants from time series of simulated records with up to four independent and identical channels even in the case of signal-to-noise ratios being as low as 2. The number of channels in a record can be determined from the dependence of the likelihood on channel number. For large enough data sets, it takes on a maximum when the assumed channel number is equal to the "true" channel number.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.