ArticleNucleic acids research1994
Sarkosyl block of transcription reinitiation by RNA polymerase II as visualized by the colliding polymerases reinitiation assay.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling.Nature communications · 2021Article
- Insight into promoter clearance by RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The capping enzyme facilitates promoter escape and assembly of a follow-on preinitiation complex for reinitiation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Investigating transcription reinitiation through in vitro approaches.Transcription · 2014Review
- Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene.PloS one · 2013Article
- Transcription initiation by human RNA polymerase II visualized at single-molecule resolution.Genes & development · 2012Article
- HDAC activity is required for efficient core promoter function at the mouse mammary tumor virus promoter.Journal of biomedicine & biotechnology · 2011Article
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.Oncogene · 2004Article
- The transcription cycle of RNA polymerase II in living cells.The Journal of cell biology · 2002Article
- Discrete promoter elements affect specific properties of RNA polymerase II transcription complexes.Nucleic acids research · 2000Article
- Multiple rounds of transcription by RNA polymerase II at covalently cross-linked templates.Nucleic acids research · 1998Article
- Functions of the N- and C-terminal domains of human RAP74 in transcriptional initiation, elongation, and recycling of RNA polymerase II.Molecular and cellular biology · 1998Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
There are indications that different concentrations of Sarkosyl can block transcription initiation by RNA polymerase II in vitro at different functional steps [Hawley and Roeder (1985) J. Biol. Chem. 260, 8163-8172]. Consequently, this reagent could be a very useful tool for mechanistic studies. So far, however, evidence for the selectivity of Sarkosyl effects on RNA polymerase II transcription has been only indirect. To directly investigate the effect of Sarkosyl on transcription initiation and reinitiation by RNA polymerase II, we employed the reinitiation assay based on utilization of templates containing G-free cassettes (colliding polymerases reinitiation assay, or CoPRA). These experiments showed unambiguously that, under the appropriate conditions, Sarkosyl can be used to block transcription reinitiation by RNA polymerase II while allowing a first round of initiations from preassembled initiation complexes. This inhibition is not due to a disruption of the SII-dependent elongation of the reinitiated transcripts, and the levels of Sarkosyl that prevent transcription reinitiation coincide with the levels that block preinitiation complex assembly. However, Sarkosyl addition to transcription reactions reconstituted with partially purified transcription factors was found to have several undesirable side effects. The usefulness and limitations of the Sarkosyl-based and CoPRA assays for measurements of transcription reinitiation are discussed.
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