ArticleNucleic acids research1994
Yeast Ty1 retrotransposon: the minus-strand primer binding site and a cis-acting domain of the Ty1 RNA are both important for packaging of primer tRNA inside virus-like particles.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Ribosome Biogenesis Modulates Ty1 Copy Number Control inGenetics · 2017Article
- Structure-Function Model for Kissing Loop Interactions That Initiate Dimerization of Ty1 RNA.Viruses · 2017Article
- Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3.Viruses · 2016Review
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription.RNA (New York, N.Y.) · 2013Article
- Exploring Ty1 retrotransposon RNA structure within virus-like particles.Nucleic acids research · 2013Article
- A succession of mechanisms stimulate efficient reconstituted HIV-1 minus strand strong stop DNA transfer.Biochemistry · 2009Article
- In vivo Ty1 reverse transcription can generate replication intermediates with untidy ends.Journal of virology · 1998Article
- Mutational analysis of the tRNA3Lys/HIV-1 RNA (primer/template) complex.Nucleic acids research · 1998Article
- Interactions between Ty1 retrotransposon RNA and the T and D regions of the tRNA(iMet) primer are required for initiation of reverse transcription in vivo.Molecular and cellular biology · 1998Article
- pCal, a highly unusual Ty1/copia retrotransposon from the pathogenic yeast Candida albicans.Journal of bacteriology · 1997Article
- Primer tRNAs for reverse transcription.Journal of virology · 1997Review
- The importance of the A-rich loop in human immunodeficiency virus type 1 reverse transcription and infectivity.Journal of virology · 1997Article
- Two step synthesis of (-) strong-stop DNA by avian and murine reverse transcriptases in vitro.Nucleic acids research · 1997Article
- Binding and kinetic properties of HIV-1 reverse transcriptase markedly differ during initiation and elongation of reverse transcription.The EMBO journal · 1996Article
- Initiation of (-) strand DNA synthesis from tRNA(3Lys) on lentiviral RNAs: implications of specific HIV-1 RNA-tRNA(3Lys) interactions inhibiting primer utilization by retroviral reverse transcriptases.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Extended interactions between the primer tRNAi(Met) and genomic RNA of the yeast Ty1 retrotransposon.Nucleic acids research · 1996Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription of the yeast retrotransposon Ty1 is primed by the cytoplasmic initiator methionine tRNA (tRNA(iMet)). The primer tRNA(iMet) is packaged inside virus-like particles (VLPs) and binds to a 10 nucleotides minus-strand primer binding site, the (-)PBS, complementary to its 3' acceptor stem. We have found that three short sequences of the Ty1 RNA (box 1, box 2.1 and box 2.2) located 3' to the (-)PBS are complementary to other regions of the primer tRNA(iMet) (T psi C and DHU stems and loops). Reconstitution of reverse transcription in vitro with T7 transcribed Ty1 RNA species and tRNA(iMet) purified from yeast cells shows that the boxes do not affect the efficiency of reverse transcription. Thus the role of the boxes on packaging of the primer tRNA(iMet) into the VLPs was investigated by analysing the level of tRNA(iMet) packaged into mutant VLPs. Specific nucleotide changes in the (-)PBS or in the boxes that do not change the protein coding sequence but disrupt the complementarity with the primer tRNA(iMet) within the VLPs. We propose that base pairing between the primer tRNA(iMet) and the Ty1 RNA is of major importance for tRNA(iMet) packaging into the VLPs. Moreover the intactness of the boxes is essential for retrotransposition as shown by the transposition defect of a Ty1 element harboring an intact (-)PBS and mutated boxes.
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