ArticleUrological research1994
Culture and characterisation of human urothelium in vivo and in vitro.
Article in Urological research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Update of the keratin gene family: evolution, tissue-specific expression patterns, and relevance to clinical disorders.Human genomics · 2022Article
- Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems.Frontiers in cellular and infection microbiology · 2021Review
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Review
- Article
- How to isolate urothelial cells? Comparison of four different methods and literature review.Human cell · 2014Review
- Differences in the epigenetic regulation of MT-3 gene expression between parental and Cd+2 or As+3 transformed human urothelial cells.Cancer cell international · 2011Article
- Beclin-1 expression in normal bladder and in Cd2+ and As3+ exposed and transformed human urothelial cells (UROtsa).Toxicology letters · 2010Article
- [Bioartificial urothelium generated from bladder washings. A future therapeutic option for reconstructive surgery].Der Urologe. Ausg. A · 2008Article
- Transformation of human urothelial cells (UROtsa) by as and cd induces the expression of keratin 6a.Environmental health perspectives · 2008Article
- [Tissue engineering and stem cell research in urology for a reconstructive or regenerative treatment approach].Der Urologe. Ausg. A · 2007Article
- Immortalization of human urothelial cells by human papillomavirus type 16 E6 and E7 genes in a defined serum-free system.Cell proliferation · 2007Article
- [In vitro stratified urothelium and its relevance in reconstructive urology].Der Urologe. Ausg. A · 2005Review
- A proliferation-dependent bystander effect in primary porcine and human urothelial explants in response to targeted irradiation.British journal of cancer · 2003Article
- Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite.Environmental health perspectives · 2002Article
- The immortalized UROtsa cell line as a potential cell culture model of human urothelium.Environmental health perspectives · 2001Article
- Cultured urothelium in sheep bladder augmentation.Pediatric surgery international · 1996Article
- Immortalisation of human urothelial cells.Urological research · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to culture human urothelium and generate enough cells for subsequent reconstructive surgery. Using a modification of the Rheinwald-Green method for the routine culture of keratinocytes from patients with burns, we successfully cultured 91% of 57 biopsies from the renal pelvis, ureter, bladder and urethra of paediatric patients. The cells could be split one to three up to 9 times at 7-10 day intervals, giving a surface area of 1000 cm2 after a 2 month culture period. Primary cultures could not be initiated in defined medium MCDB153, although cells initiated using the Rheinwald-Green method could subsequently be propagated in this medium. Cytokeratin patterns in vitro were similar to those in vivo in the expression of keratins 7, 18 and 19 (characteristic of simple epithelia) and keratin 13 (characteristic of non-cornified stratified epithelia). Cultured urothelium also expressed keratin 14 (characteristic of cornified stratified epithelium) in about 25% of cells and keratin 16 (characteristic of fast-growing cells). These findings indicate that urothelial cells can be propagated in vitro for autologous grafting, and the next step is to identify substrates suitable for urothelial cell growth and differentiation and surgical manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.