ArticleNucleic acids research1994
Interactions among regulators of RNA abundance characterized using RNA fingerprinting by arbitrarily primed PCR.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 45 citations in OpenAlex.
- Assessment of gene expression in many samples using vertical arrays.Nucleic acids research · 2008Article
- Enhanced microarray performance using low complexity representations of the transcriptome.Nucleic acids research · 2005Article
- Detection of differentially expressed genes in an isogenic breast metastasis model using RNA arbitrarily primed-polymerase chain reaction coupled with array hybridization (RAP-array).The American journal of pathology · 2004Article
- Non-stoichiometric reduced complexity probes for cDNA arrays.Nucleic acids research · 1998Article
- Cloning and characterization of a putative human serine/threonine protein kinase transcriptionally modified during anisotonic and isotonic alterations of cell volume.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Differential display of intestinal mRNAs regulated by dietary zinc.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- DNA rehybridization during PCR: the 'Cot effect' and its consequences.Nucleic acids research · 1996Article
- Screening of differentially amplified cDNA products from RNA arbitrarily primed PCR fingerprints using single strand conformation polymorphism (SSCP) gels.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Using RNA fingerprinting by arbitrarily primed PCR it is possible to infer convergent transcript regulatory pathways from the coordinate behavior of subsets of anonymous transcripts without cloning any genes. The number of transcripts in each response category can be estimated. The same may be true for differential display. We demonstrate these claims by treating a cell line with two known modulators of RNA abundance, transforming growth factor-beta (TGF beta) and cycloheximide (CX), used together and alone. The responses of over 1700 anonymous transcripts were monitored under these three conditions and in an untreated control. Eight of the twenty-seven [3(3)] possible transcript response categories were observed among 86 differentially expressed transcripts. For example, CX stabilizes or induces as many as 2.7% of transcripts of which about one third do not accumulate when TGF beta is also present. This intersection may reflect CX stabilization or induction of an important class of RNAs that otherwise usually have short half-lives. We predict that RNAs in this class constitute the majority of transcripts targeted for rapid down regulation in response to TGF beta and perhaps most other natural transcriptional modulators.
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