ArticleMolecular and cellular biology1994
Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 94 citations in OpenAlex.
- Article
- ISGylation is induced in neurons by demyelination driving ISG15-dependent microglial activation.Journal of neuroinflammation · 2022Article
- Article
- ISGylation Inhibits an LPS-Induced Inflammatory Response via the TLR4/NF-κB Signaling Pathway in Goat Endometrial Epithelial Cells.Animals : an open access journal from MDPI · 2021Article
- Airway basal cell injury after acute diacetyl (2,3-butanedione) vapor exposure.Toxicology letters · 2020Article
- Expression Analysis of Interferon-Stimulated Gene 15 in the Rock BreamDevelopment & reproduction · 2017Article
- Free ISG15 triggers an antitumor immune response against breast cancer: a new perspective.Oncotarget · 2015Article
- The human oviduct transcriptome reveals an anti-inflammatory, anti-angiogenic, secretory and matrix-stable environment during embryo transit.Reproductive biomedicine online · 2013Article
- The role of the ubiquitin proteasome pathway in keratin intermediate filament protein degradation.Proceedings of the American Thoracic Society · 2010Review
- ISG15 as a novel tumor biomarker for drug sensitivity.Molecular cancer therapeutics · 2008Article
- HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Down-regulation of UCRP and UBE2L6 in BRCA2 knocked-down human breast cells.Biochemical and biophysical research communications · 2005Article
- The 14-3-3 protein epsilon isoform expressed in reactive astrocytes in demyelinating lesions of multiple sclerosis binds to vimentin and glial fibrillary acidic protein in cultured human astrocytes.The American journal of pathology · 2004Article
- Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins.Molecular and cellular biology · 2004Article
- Searching for interferon-induced genes that inhibit hepatitis B virus replication in transgenic mouse hepatocytes.Journal of virology · 2003Article
- Deubiquitinating function of adenovirus proteinase.Journal of virology · 2002Article
- Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein.The EMBO journal · 2001Article
- The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.Molecular and cellular biology · 2000Article
- Regulation of double-strand break-induced mammalian homologous recombination by UBL1, a RAD51-interacting protein.Nucleic acids research · 2000Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Ubiquitin cross-reactive protein (UCRP), a 15-kDa interferon-induced protein, is a sequence homolog of ubiquitin that is covalently ligated to intracellular proteins in a parallel enzymatic reaction and is found at low levels within cultured cell lines and human tissues not exposed to interferon. Ubiquitin and UCRP ligation reactions apparently target distinct subsets of intracellular proteins, as judged from differences in the distributions of the respective adducts revealed on immunoblots. In this study, successive passages of the human lung carcinoma line A549 in the presence of neutralizing antibodies against alpha and beta interferons had no effect on the levels of either free or conjugated UCRP, indicating that these UCRP pools are constitutively present within uninduced cells and are thus not a consequence of autoinduction by low levels of secreted alpha/beta interferon. In an effort to identify potential targets for UCRP conjugation, the immunocytochemical distribution of UCRP was examined by using affinity-purified polyclonal antibodies against recombinant polypeptide. UCRP distributes in a punctate cytoskeletal pattern that is resistant to extraction by nonionic detergents (e.g., Triton X-100) in both uninduced and interferon-treated A549 cells. The cytoskeletal pattern colocalizes with the intermediate filament network of epithelial and mesothelial cell lines. Immunoblots of parallel Triton X-100-insoluble cell extracts suggest that the cytoskeletal association largely results from the noncovalent association of UCRP conjugates with the intermediate filaments rather than direct ligation of the polypeptide to structural components of the filaments. A significant increase in the sequestration of UCRP adducts on intermediate filaments accompanies interferon induction. These results suggest that UCRP may serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments.
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