ArticleThe Journal of cell biology1994
Expression of beta 1B integrin isoform in CHO cells results in a dominant negative effect on cell adhesion and motility.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
- mTOR pathway inhibition alters proliferation as well as differentiation of neural stem cells.Frontiers in cellular neuroscience · 2024Article
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- The novel α4B murine α4 integrin protein splicing variant inhibits α4 protein-dependent cell adhesion.The Journal of biological chemistry · 2014Article
- C-terminal COOH of integrin β1 is necessary for β1 association with the kindlin-2 adapter protein.The Journal of biological chemistry · 2014Article
- A novel mutation in β integrin reveals an integrin-mediated interaction between the extracellular matrix and cki-1/p27KIP1.PloS one · 2012Article
- Extended interaction of beta1 integrin subunit-deficient cells (GD25) with surfaces modified with fibronectin-derived peptides: Culture optimization, adhesion and cytokine panel studies.Acta biomaterialia · 2008Article
- Disruption of C-terminal cytoplasmic domain of betaPS integrin subunit has dominant negative properties in developing Drosophila.Molecular biology of the cell · 2002Article
- The integrin cytoplasmic domain-associated protein ICAP-1 binds and regulates Rho family GTPases during cell spreading.The Journal of cell biology · 2002Article
- Cross talk between beta(1) and alpha(V) integrins: beta(1) affects beta(3) mRNA stability.Molecular biology of the cell · 2001Article
- Contact with fibrillar collagen inhibits melanoma cell proliferation by up-regulating p27KIP1.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Focal adhesion targeting: the critical determinant of FAK regulation and substrate phosphorylation.Molecular biology of the cell · 1999Article
- R-Ras signals through specific integrin alpha cytoplasmic domains to promote migration and invasion of breast epithelial cells.The Journal of cell biology · 1999Article
- Article
- Beta1C integrin in epithelial cells correlates with a nonproliferative phenotype: forced expression of beta1C inhibits prostate epithelial cell proliferation.The American journal of pathology · 1998Article
- Interactions between growth factors and integrins: latent forms of transforming growth factor-beta are ligands for the integrin alphavbeta1.Molecular biology of the cell · 1998Article
- beta1-integrin cytoplasmic subdomains involved in dominant negative function.Molecular biology of the cell · 1998Article
- Identification of beta1C-2, a novel variant of the integrin beta1 subunit generated by utilization of an alternative splice acceptor site in exon C.The Biochemical journal · 1998Article
- Muscle beta1D integrin reinforces the cytoskeleton-matrix link: modulation of integrin adhesive function by alternative splicing.The Journal of cell biology · 1997Article
- Dominant-negative effect on adhesion by myelin Po protein truncated in its cytoplasmic domain.The Journal of cell biology · 1996Article
- Down-regulation of beta 1C integrin, an inhibitor of cell proliferation, in prostate carcinoma.The American journal of pathology · 1996Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The integrin subunit beta 1B, a beta 1 isoform with a unique sequence at the cytoplasmic domain, forms heterodimers with integrin alpha chains and binds fibronectin, but it does not localize to focal adhesion sites (Balzac, F., A. Belkin, V. Koteliansky, Y. Balabanow, F. Altruda, L. Silengo, and G. Tarone. 1993. J. Cell Biol. 121:171-178). Here we analyze the functional properties of human beta 1B by expressing it in hamster CHO cells. When stimulated by specific antibodies, beta 1B does not trigger tyrosine phosphorylation of a 125-kD cytosolic protein, an intracellular signalling pathway that is activated both by the endogenous hamster or the transfected human beta 1A. Moreover, expression of beta 1B results in reduced spreading on fibronectin and laminin, but not on vitronectin. Expression of beta 1B also results in severe reduction of cell motility in the Boyden chamber assay. Reduced cell spreading and motility could not be accounted for by preferential association of beta 1B with a given integrin alpha subunit. These data, together with our previous results, indicate that beta 1B interferes with beta 1A function when expressed in CHO cells resulting in a dominant negative effect on cell adhesion and migration.
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