Evidence map›Paper›PMID 7523337›Full record

ArticleHistochemistry1994

Reflection contrast microscopy within chrome-alum haematoxylin stained thick tissue-sections.

T J Filler, C H Rickert, U K Fassnacht, F Pera

Abstract read
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In one paragraph

Article in Histochemistry, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

T J FillerInstitute of Anatomy, Westfälische Wilhelms-University, Münster, Germany.
C H Rickert
U K Fassnacht
F Pera

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This paper introduces two innovations in reflection contrast microscopy (RCM): (1) an extended application for qualitative light microscopic investigations; and (2) a novel method for quantification in cytochemistry. (1) We found out that RCM cannot only be used for surface characterizations and in thin sections but also within thick tissue-sections. The use of the RCM technique is demonstrated on slides of the supraoptic nucleus (SON) of the rat stained with chrome-alum haematoxylin: Among all the stained structures only neurosecretory granules are found to cause reflections. The visualization of the neurosecretion and its distribution is more distinct and of sharper contrast than in bright field microscopy. (2) The improved differentiation allows the quantification of neurosecretion in tissue-sections by combining RCM with grey-scale image analysis.

Indexed as

AnimalsBrainChromium CompoundsColoring AgentsHematoxylinHistocytochemistryImage Processing, Computer-AssistedMaleMicroscopyPotassium CompoundsRatsRats, WistarStaining and LabelingSulfatesSupraoptic Nucleuschrome alumChromium CompoundsColoring AgentsHematoxylinPotassium CompoundsSulfates

Identifiers

PMID7523337

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.