ArticleInfection and immunity1994
A surface epitope undergoing high-frequency phase variation is shared by Mycoplasma gallisepticum and Mycoplasma bovis.
Article in Infection and immunity, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- Review
- Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA.Journal, genetic engineering & biotechnology · 2022Article
- Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum.Poultry science · 2020Review
- Article
- A novel transposon construct expressing PhoA with potential for studying protein expression and translocation in Mycoplasma gallisepticum.BMC microbiology · 2012Article
- Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum.Infection and immunity · 2010Article
- Avoidance of the host immune system through phase variation in Mycoplasma pulmonis.Infection and immunity · 2005Article
- Phase and antigenic variation in bacteria.Clinical microbiology reviews · 2004Review
- Phenotypic switching in Mycoplasma gallisepticum hemadsorption is governed by a high-frequency, reversible point mutation.Infection and immunity · 2003Article
- Juxtaposition of an active promoter to vsp genes via site-specific DNA inversions generates antigenic variation in Mycoplasma bovis.Journal of bacteriology · 2001Article
- Characterization and analysis of a stable serotype-associated membrane protein (P30) of Mycoplasma agalactiae.Journal of clinical microbiology · 2001Article
- Molecular variability of the adhesin-encoding gene pvpA among Mycoplasma gallisepticum strains and its application in diagnosis.Journal of clinical microbiology · 2001Article
- pMGA phenotypic variation in Mycoplasma gallisepticum occurs in vivo and is mediated by trinucleotide repeat length variation.Infection and immunity · 2000Article
- In vitro cell invasion of Mycoplasma gallisepticum.Infection and immunity · 2000Article
- Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein.Infection and immunity · 2000Article
- P48 major surface antigen of Mycoplasma agalactiae is homologous to a malp product of Mycoplasma fermentans and belongs to a selected family of bacterial lipoproteins.Infection and immunity · 1999Article
- The vsp locus of Mycoplasma bovis: gene organization and structural features.Journal of bacteriology · 1999Article
- Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin.Infection and immunity · 1997Article
- Cloning and functional analysis of the P97 swine cilium adhesin gene of Mycoplasma hyopneumoniae.Journal of bacteriology · 1997Article
- Phenotypic switching of variable surface lipoproteins in Mycoplasma bovis involves high-frequency chromosomal rearrangements.Journal of bacteriology · 1996Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently reported that three distinct size- and phase-variable surface lipoproteins (Vsps) of the bovine pathogen Mycoplasma bovis possess a common epitope recognized by monoclonal antibody 1E5. In the present study, we show that this epitope is also present on a size-variant protein (PvpA) of the avian pathogen Mycoplasma gallisepticum. Application of monoclonal antibody 1E5 in Western immunoblot analysis of Triton X-114 phase-fractionated proteins and in colony immunoblots, as well as in trypsin and carboxypeptidase digestion experiments, has demonstrated that (i) PvpA is an integral membrane protein with a free C terminus, (ii) the shared epitope is surface exposed, and (iii) PvpA is subjected to high-frequency phase variation in expression. By using serum antibodies from M. gallisepticum-infected chickens, we were able to demonstrate the immunogenic nature of PvpA and identify three additional highly immunogenic Triton X-114 phase proteins (p67, p72, and p75) also undergoing high-frequency phase variation spontaneously and independently. Metabolic labeling experiments with [14C]palmitate and [14C]oleate revealed that PvpA, in contrast to p67, p72, and p75, is not lipid modified. Southern blot hybridization with restriction fragments carrying the pvpA gene of M. gallisepticum or the vspA gene of M. bovis against digested genomic DNA of the two Mycoplasma species indicated the absence of genetic relatedness between the pvpA and vspA genes. The apparent complexity of the antigenic variation phenomenon in M. gallisepticum is discussed.
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