ArticleBiophysical journal1994
Improved technique for studying ion channels expressed in Xenopus oocytes, including fast superfusion.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- A mechanism for graded motor control encoded in the channel properties of the muscle ACh receptor.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- High throughput electrophysiology with Xenopus oocytes.Combinatorial chemistry & high throughput screening · 2009Review
- A human congenital myasthenia-causing mutation (epsilon L78P) of the muscle nicotinic acetylcholine receptor with unusual single channel properties.The Journal of physiology · 2005Article
- Position 170 of Rabbit Na+/glucose cotransporter (rSGLT1) lies in the Na+ pathway; modulation of polarity/charge at this site regulates charge transfer and carrier turnover.Biophysical journal · 2004Article
- Regulation of Kir channels by intracellular pH and extracellular K(+): mechanisms of coupling.The Journal of general physiology · 2004Article
- Ionic currents in the human serotonin transporter reveal inconsistencies in the alternating access hypothesis.Biophysical journal · 2003Article
- Investigating the conformational states of the rabbit Na+/glucose cotransporter.Biophysical journal · 2003Article
- Activation and desensitization of the recombinant P2X1 receptor at nanomolar ATP concentrations.The Journal of general physiology · 2003Article
- Permeant cations and blockers modulate pH gating of ROMK channels.Biophysical journal · 2003Article
- Electrostatic interaction between charybdotoxin and a tetrameric mutant of Shaker K(+) channels.Biophysical journal · 2000Article
- Feedback inhibition of rat amiloride-sensitive epithelial sodium channels expressed in Xenopus laevis oocytes.The Journal of physiology · 1999Article
- Ca2+ permeability of mouse and chick nicotinic acetylcholine receptors expressed in transiently transfected human cells.The Journal of physiology · 1998Article
- Control of NMDA receptor activation by a glycine transporter co-expressed in Xenopus oocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Rapid chemical kinetic techniques for investigations of neurotransmitter receptors expressed in Xenopus oocytes.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Fast voltage clamp discloses a new component of presteady-state currents from the Na(+)-glucose cotransporter.Biophysical journal · 1996Article
- Dependence of solution exchange time on cell or patch linear dimensions in concentration jump experiments using patch-clamped sensory neurones.Pflugers Archiv : European journal of physiology · 1996Article
- Thermodynamic determination of the Na+: glucose coupling ratio for the human SGLT1 cotransporter.Biophysical journal · 1995Article
- Effects of serotonergic agents on neuronal nicotinic acetylcholine receptors.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Comparison of quantitative calcium flux through NMDA, ATP, and ACh receptor channels.Biophysical journal · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of whole-cell currents from ion channels expressed in Xenopus oocytes with conventional two-electrode voltage clamp has two major limitations. First, the large diameter and spherical geometry of oocytes prevent extremely fast solution changes. Second, the internal medium is not controlled, which limits the experimental versatility of the oocyte expression system. For example, because the internal medium is not controlled, endogenous calcium-activated chloride conductances can contaminate currents measured with channels that are permeable to calcium. We describe a new technique that combines vaseline-gap voltage clamp for oocytes with a fast superfusion system. The vaseline-gap procedure is simplified by having the micropipette that monitors voltage serve a dual role as a perfusion micropipette that controls the internal solution. In addition, the technique provides fast external solution changes that are complete in 30-50 ms. We applied the approach to measure the calcium permeability of a muscle and a neuronal nicotinic acetylcholine receptor. Very fast agonist induced currents were measured without contamination by the secondary activation of calcium-dependent chloride channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.