ArticleMolecular and cellular biology1994
Ty1 in vitro integration: effects of mutations in cis and in trans.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity in vitro.The Journal of biological chemistry · 2021Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Identification and characterization of critical cis-acting sequences within the yeast Ty1 retrotransposon.RNA (New York, N.Y.) · 2005Article
- Correct integration of model substrates by Ty1 integrase.Journal of virology · 2000Article
- Reverse transcription of a self-primed retrotransposon requires an RNA structure similar to the U5-IR stem-loop of retroviruses.Molecular and cellular biology · 1998Article
- Article
- Invading the yeast nucleus: a nuclear localization signal at the C terminus of Ty1 integrase is required for transposition in vivo.Molecular and cellular biology · 1998Article
- A Ty1 integrase nuclear localization signal required for retrotransposition.Molecular and cellular biology · 1998Article
- Retrovirus-like end processing of the tobacco Tnt1 retrotransposon linear intermediates of replication.Journal of virology · 1997Article
- A critical proteolytic cleavage site near the C terminus of the yeast retrotransposon Ty1 Gag protein.Journal of virology · 1996Article
- Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA.Journal of virology · 1996Article
- Substrate specificity of Ty1 integrase.Journal of virology · 1995Article
- In vitro integration of retrotransposon Ty1: a direct physical assay.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mutations within the TYB gene of Ty1 encoding integrase (IN) as well as alterations in its substrate, a linear DNA molecule, were examined for their effects on in vitro IN activity, using a recently developed physical assay. Five different codon-insertion mutations, two frameshift mutations, and one missense mutation, previously identified as transposition-deficient mutations, were tested. Virus-like particles, the source of IN, from two different protease mutants and a reverse transcriptase mutant exhibited near-normal to normal IN activity. Two frameshift mutations mapping within the phylogenetically variable C-terminal domain of IN resulted in significant in vitro IN activity. In contrast, three mutations within the amino-terminal conserved domain of IN completely abolished IN activity. When the substrate termini were mutated, we found that substrates with as few as 4 bp of Ty1 termini were capable of efficiently generating integration products. Surprisingly, certain substrates that lacked obvious similarity to Ty1 termini were also readily integrated into both linear and circular targets, whereas others were not used as substrates at all. Termini rich in adenosine residues were among the more active substrates; however, certain substrates lacking terminal adenosine residues can form small quantities of integration products, including complete integration reactions.
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