ArticleChromosoma1994
Generation and characterization of novel antibodies highly selective for phosphorylated linker histone H1 in Tetrahymena and HeLa cells.
Article in Chromosoma, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
- Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation.Epigenetics & chromatin · 2017Article
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- DUX4 induces a transcriptome more characteristic of a less-differentiated cell state and inhibits myogenesis.Journal of cell science · 2016Article
- DNA copy-number control through inhibition of replication fork progression.Cell reports · 2014Article
- The molecular chaperone Hsp90 is required for cell cycle exit in Drosophila melanogaster.PLoS genetics · 2013Article
- Chromatin unfolding by Cdt1 regulates MCM loading via opposing functions of HBO1 and HDAC11-geminin.Cell cycle (Georgetown, Tex.) · 2010Article
- Histone H1 phosphorylation is associated with transcription by RNA polymerases I and II.The Journal of cell biology · 2010Article
- Article
- cAMP signaling induces rapid loss of histone H3 phosphorylation in mammary adenocarcinoma-derived cell lines.Experimental cell research · 2008Article
- Article
- Regulation of hypermutation by activation-induced cytidine deaminase phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Regulation of replication licensing by acetyltransferase Hbo1.Molecular and cellular biology · 2006Article
- A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytes.Genes & development · 2005Article
- Chromatin decondensation in S-phase involves recruitment of Cdk2 by Cdc45 and histone H1 phosphorylation.The Journal of cell biology · 2005Article
- The dynamic mobility of histone H1 is regulated by cyclin/CDK phosphorylation.Molecular and cellular biology · 2003Article
- Article
- Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila.Molecular biology of the cell · 1999Article
- Article
- An HP1-like protein is missing from transcriptionally silent micronuclei of Tetrahymena.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Entry into mitosis in vertebrate somatic cells is guarded by a chromosome damage checkpoint that reverses the cell cycle when triggered during early but not late prophase.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Phosphorylated forms of Tetrahymena macronuclear histone H1 were separated from each other and from dephosphorylated H1 by cation-exchange HPLC. A homogeneous fraction of hyperphosphorylated macronuclear H1 was then used to generate novel polyclonal antibodies highly selective for phosphorylated H1 in Tetrahymena and in human cells. These antibodies fail to recognize dephosphorylated forms of H1 in both organisms and are not reactive with most other nuclear or cytoplasmic phosphoproteins including those induced during mitosis. The selectivity of these antibodies for phosphorylated forms of H1 in Tetrahymena and in HeLa argues strongly that these antibodies recognize highly conserved phosphorylated epitopes found in most H1s and from this standpoint Tetrahymena H1 is not atypical. Using these antibodies in indirect immunofluorescence analyses, we find that a significant fraction of interphase mammalian cells display a strikingly punctate pattern of nuclear fluorescence. As cells enter S-phase, nuclear staining becomes more diffuse, increases significantly, and continues to increase as cells enter mitosis. As cells exit from mitosis, staining with the anti-phosphorylated H1 antibodies is rapidly lost presumably owing to the dephosphorylation of H1. These immunofluorescent data document precisely the cell cycle changes in the level of H1 phosphorylation determined by earlier biochemical studies and suggest that these antibodies represent a powerful new tool to probe the function(s) of H1 phosphorylation in a wide variety of eukaryotic systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.