ArticleThe Journal of cell biology1994
Dictyostelium discoideum mutants with conditional defects in phagocytosis.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- A 'dynamic adder model' for cell size homeostasis in Dictyostelium cells.Scientific reports · 2021Article
- Identification of a Chemoattractant G-Protein-Coupled Receptor for Folic Acid that Controls Both Chemotaxis and Phagocytosis.Developmental cell · 2016Article
- Horizontal gene transfer in chromalveolates.BMC evolutionary biology · 2007Article
- Origin of phagotrophic eukaryotes as social cheaters in microbial biofilms.Biology direct · 2007Article
- Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system.Journal of bacteriology · 2002Article
- G protein beta subunit-null mutants are impaired in phagocytosis and chemotaxis due to inappropriate regulation of the actin cytoskeleton.The Journal of cell biology · 1998Article
- Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis.The Journal of cell biology · 1997Article
- Evidence for a recycling role for Rab7 in regulating a late step in endocytosis and in retention of lysosomal enzymes in Dictyostelium discoideum.Molecular biology of the cell · 1997Article
- Actin-, myosin- and ubiquitin-dependent endocytosis.Experientia · 1996Review
- A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.The Journal of cell biology · 1996Article
- Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants.Molecular biology of the cell · 1996Article
- Dictyostelium mutants lacking multiple classic myosin I isoforms reveal combinations of shared and distinct functions.The Journal of cell biology · 1996Article
- Cell adhesion in the life cycle of Dictyostelium.Experientia · 1995Review
- The Dictyostelium cytoskeleton.Experientia · 1995Review
- Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis.The Journal of cell biology · 1995Article
- Genetic and physiologic modulation of the prestarvation response in Dictyostelium discoideum.Molecular biology of the cell · 1995Article
- Dictyostelium discoideum mutants with temperature-sensitive defects in endocytosis.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have isolated and characterized Dictyostelium discoideum mutants with conditional defects in phagocytosis. Under suspension conditions, the mutants exhibited dramatic reductions in the uptake of bacteria and polystyrene latex beads. The initial binding of these ligands was unaffected, however, indicating that the defect was not in a plasma membrane receptor: Because of the phagocytosis defect, the mutants were unable to grow when cultured in suspensions of heat-killed bacteria. The mutants exhibited normal capacities for fluid phase endocytosis and grew as rapidly as parental (AX4) cells in axenic medium. Both the defects in phagocytosis and growth on bacteria were corrected when the mutant Dictyostelium cells were cultured on solid substrates. Reversion and genetic complementation analysis suggested that the mutant phenotypes were caused by single gene defects. While the precise site of action of the mutations was not established, the mutations are likely to affect an early signaling event because the binding of bacteria to mutant cells in suspension was unable to trigger the localized polymerization of actin filaments required for ingestion; other aspects of actin function appeared normal. This class of conditional phagocytosis mutant should prove to be useful for the expression cloning of the affected gene(s).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.