ArticleThe Journal of cell biology1994
Stable expression of heterologous microtubule-associated proteins (MAPs) in Chinese hamster ovary cells: evidence for differing roles of MAPs in microtubule organization.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- New genes associated with schizophrenia in neurite formation: a review of cell culture experiments.Molecular psychiatry · 2007Pooled it
- Sequences in the stalk domain regulate auto-inhibition and ciliary tip localization of the immotile kinesin-4 KIF7.Journal of cell science · 2021Article
- The role of microtubules and their dynamics in cell migration.The Journal of biological chemistry · 2012Article
- New insights into mechanisms of resistance to microtubule inhibitors.Biochimica et biophysica acta · 2011Review
- Class V β-tubulin alters dynamic instability and stimulates microtubule detachment from centrosomes.Molecular biology of the cell · 2011Article
- Megakaryocyte lineage-specific class VI β-tubulin suppresses microtubule dynamics, fragments microtubules, and blocks cell division.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs.The Journal of biological chemistry · 2010Article
- Molecular basis for class V beta-tubulin effects on microtubule assembly and paclitaxel resistance.The Journal of biological chemistry · 2009Article
- The individualization of cancer therapy: the unexpected role of p53.Transactions of the American Clinical and Climatological Association · 2006Review
- The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.Molecular and cellular biology · 2005Article
- Protein kinase MARK/PAR-1 is required for neurite outgrowth and establishment of neuronal polarity.Molecular biology of the cell · 2002Article
- PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone.The Journal of cell biology · 2002Article
- Stable expression in Chinese hamster ovary cells of mutated tau genes causing frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17).The American journal of pathology · 1999Article
- The Opitz syndrome gene product, MID1, associates with microtubules.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The development of cell processes induced by tau protein requires phosphorylation of serine 262 and 356 in the repeat domain and is inhibited by phosphorylation in the proline-rich domains.Molecular biology of the cell · 1999Article
- Overexpression of tau protein inhibits kinesin-dependent trafficking of vesicles, mitochondria, and endoplasmic reticulum: implications for Alzheimer's disease.The Journal of cell biology · 1998Article
- Acute inactivation of tau has no effect on dynamics of microtubules in growing axons of cultured sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Microtubule stabilization in pressure overload cardiac hypertrophy.The Journal of cell biology · 1997Article
- Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Regulated phosphorylation and dephosphorylation of tau protein: effects on microtubule interaction, intracellular trafficking and neurodegeneration.The Biochemical journal · 1997Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To study the effects of microtubule-associated proteins (MAPs) on in vivo microtubule assembly, cDNAs containing the complete coding sequences of a Drosophila 205-kD heat stable MAP, human MAP 4, and human tau were stably transfected into CHO cells. Constitutive expression of the transfected genes was low in most cases and had no obvious effects on the viability of the transfected cell lines. High levels of expression, as judged by Western blots, immunofluorescence, and Northern blots, could be induced by treating cells with sodium butyrate. High levels of MAPs were maintained for at least 24-48 h after removal of the sodium butyrate. Immunofluorescence analysis indicated that all three MAPs bound to cellular microtubules, but only the transfected tau caused a rearrangement of microtubules into bundles. Despite high levels of expression of these exogenous MAPs and the bundling of microtubules in cells expressing tau, transfected cells had normal levels of assembled and unassembled tubulin. With the exception of the tau-induced bundles, microtubules in transfected cells showed the same sensitivity as control cells to microtubule depolymerization by Colcemid. Further, all three MAPs were ineffective in reversing the taxol-dependent phenotype of a CHO mutant cell line. The absence of a quantitative effect of any of these heterologous proteins on the assembly of tubulin suggests that these MAPs may have different roles in vivo from those inferred previously from in vitro experiments.
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