ArticleThe EMBO journal1994
Functional coupling of a Ca2+/calmodulin-dependent nitric oxide synthase and a soluble guanylyl cyclase in vertebrate photoreceptor cells.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 162 citations in OpenAlex.
- Article
- Retinal Physiology and Circulation: Effect of Diabetes.Comprehensive Physiology · 2020Review
- Control of the Nucleotide Cycle in Photoreceptor Cell Extracts by Retinal Degeneration Protein 3.Frontiers in molecular neuroscience · 2018Article
- Regulation of Neuronal Oxygen Responses in C. elegans Is Mediated through Interactions between Globin 5 and the H-NOX Domains of Soluble Guanylate Cyclases.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Article
- Nitric oxide potentiates TNF-α-induced neurotoxicity through suppression of NF-κB.Cellular and molecular neurobiology · 2012Article
- Imaging of nitric oxide in the retina.Vision research · 2005Article
- Nitric oxide and octreotide in retinal ischemia-reperfusion injury.Documenta ophthalmologica. Advances in ophthalmology · 2002Article
- Calcium regulation in photoreceptors.Frontiers in bioscience : a journal and virtual library · 2002Review
- Ca(2+) sensor S100beta-modulated sites of membrane guanylate cyclase in the photoreceptor-bipolar synapse.The EMBO journal · 2002Article
- Calcium and retinal function.Molecular neurobiology · 2002Review
- Calcium-modulated membrane guanylate cyclase in synaptic transmission?Molecular and cellular biochemistry · 2002Review
- Soluble guanylate cyclases in the retina.Molecular and cellular biochemistry · 2002Review
- Ligand sensitivity of the 2 subunit from the bovine cone cGMP-gated channel is modulated by protein kinase C but not by calmodulin.The Journal of physiology · 2001Article
- Modulation of the light response by cAMP in Drosophila photoreceptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Compartmentalization of calcium extrusion mechanisms in the outer and inner segments of photoreceptors.Neuron · 1998Article
- Calmodulin controls the rod photoreceptor CNG channel through an unconventional binding site in the N-terminus of the beta-subunit.The EMBO journal · 1998Article
- Mechanisms of cell death in the inherited retinal degenerations.American journal of human genetics · 1998Review
- Nitric oxide synthase activity in tissues of the bovine eye.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 1997Article
- Cytochemical localization of guanylate cyclase in photoreceptor cells of the mouse.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrophysiological recordings on retinal rod cells, horizontal cells and on-bipolar cells indicate that exogenous nitric oxide (NO) has neuromodulatory effects in the vertebrate retina. We report here endogenous NO formation in mammalian photoreceptor cells. Photoreceptor NO synthase resembled the neuronal NOS type I from mammalian brain. NOS activity utilized the substrate L-arginine (Km = 4 microM) and the cofactors NADPH, FAD, FMN and tetrahydrobiopterin. The activity showed a complete dependence on the free calcium concentration ([Ca2+]) and was mediated by calmodulin. NO synthase activity was sufficient to activate an endogenous soluble guanylyl cyclase that copurified in photoreceptor preparations. This functional coupling was strictly controlled by the free [Ca2+] (EC50 = 0.84 microM). Activation of the soluble guanylyl cyclase by endogenous NO was up to 100% of the maximal activation of this enzyme observed with the exogenous NO donor compound sodium nitroprusside. This NO/cGMP pathway was predominantly localized in inner and not in outer segments of photoreceptors. Immunocytochemically, we localized NO synthase type I mainly in the ellipsoid region of the inner segments and a soluble guanylyl cyclase in cell bodies of cone photoreceptor cells. We conclude that in photoreceptors endogenous NO is functionally coupled to a soluble guanylyl cyclase and suggest that it has a neuromodulatory role in visual transduction and in synaptic transmission in the outer retina.
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