ArticleMolecular and cellular biology1994
GATA-binding proteins regulate the human gonadotropin alpha-subunit gene in the placenta and pituitary gland.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 183 citations in OpenAlex.
- Signal Amplification in the HPT Axis-Evidence for Its Existence, Location, Significance, and Molecular Mechanisms.Acta physiologica (Oxford, England) · 2026Review
- Aberrant trophoblastic differentiation in human cancer: An emerging novel therapeutic target (Review).Oncology reports · 2024Review
- Article
- Regulation of human trophoblast syncytialization by histone demethylase LSD1.The Journal of biological chemistry · 2019Article
- Review
- Chromatin status and transcription factor binding to gonadotropin promoters in gonadotrope cell lines.Reproductive biology and endocrinology : RB&E · 2017Article
- Proximal GATA-binding sites are essential for human HSD3B1 gene transcription in the placenta.Scientific reports · 2017Article
- The Arcuate Estrogen-Regulated Transcriptome: Estrogen Response Element-Dependent and -Independent Signaling of ERα in Female Mice.Endocrinology · 2017Article
- Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-B, specificity protein (SP)1-like and GATA-4 binding sites.Reproductive biology and endocrinology : RB&E · 2016Article
- GATA factors in endocrine neoplasia.Molecular and cellular endocrinology · 2016Review
- Essential Role of GATA2 in the Negative Regulation of Type 2 Deiodinase Gene by Liganded Thyroid Hormone Receptor β2 in Thyrotroph.PloS one · 2015Article
- GATA augments GNRH-mediated increases in Adcyap1 gene expression in pituitary gonadotrope cells.Journal of molecular endocrinology · 2013Article
- Cell-specific actions of a human LHX3 gene enhancer during pituitary and spinal cord development.Molecular endocrinology (Baltimore, Md.) · 2013Article
- GATA Transcription Factors in Pregnancy.Medical journal of obstetrics and gynecology · 2013Article
- Detailed morphogenetic analysis of the embryonic chicken pars tuberalis as glycoprotein alpha subunit positive region.Journal of molecular histology · 2013Article
- Msx1 homeodomain protein represses the αGSU and GnRH receptor genes during gonadotrope development.Molecular endocrinology (Baltimore, Md.) · 2013Article
- GATA2-induced silencing and LIM-homeodomain protein-induced activation are mediated by a bi-functional response element in the rat GnRH receptor gene.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Expression and potential role of GATA factors in trophoblast development.The Journal of reproduction and development · 2013Review
- The HMG-box transcription factor Sox4b is required for pituitary expression of gata2a and specification of thyrotrope and gonadotrope cells in zebrafish.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Squelching of ETS2 transactivation by POU5F1 silences the human chorionic gonadotropin CGA subunit gene in human choriocarcinoma and embryonic stem cells.Molecular endocrinology (Baltimore, Md.) · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The human glycoprotein hormone alpha-subunit gene is expressed in two quite dissimilar tissues, the placenta and anterior pituitary. Tissue-specific expression is determined by combinations of elements, some of which are common and others of which are specific to each tissue. In the placenta, a composite enhancer confers specific expression. It contains four protein-binding sites: two cyclic AMP (cAMP) response elements that bind CREB, a trophoblast-specific element that binds TSEB, and a sequence motif, AGATAA, that matches the consensus binding site for a family of transcription factors termed the GATA-binding proteins. In pituitary gonadotropes, the cAMP response elements remain important for expression, TSEB is absent, and elements further upstream participate in tissue-specific expression. Here we establish a regulatory role for the GATA element in both the placenta and pituitary by demonstrating that a mutation of this element decreases alpha-subunit gene expression 15-fold in JEG-3 human placental cells and 2.5-fold in alpha T3-1 mouse pituitary gonadotropes. In JEG-3 cells, human GATA-2 (hGATA-2) and hGATA-3 are highly expressed and both proteins bind to the alpha-subunit gene GATA element. In alpha T3-1 cells, the GATA motif is bound by mouse GATA-2 (mGATA-2) and an mGATA-4-related protein. Cotransfection of hGATA-2 or hGATA-3 into alpha T3-1 cells activates the alpha-subunit gene threefold. These studies establish a role for the GATA-binding proteins in placental and pituitary alpha-subunit gene expression, significantly expanding the known target genes of GATA-2, GATA-3, and perhaps GATA-4.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.