ArticleJournal of bacteriology1994
Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Components Subcellular Localization: Cell Surface Exposure.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Outer membrane lipoprotein biogenesis: Lol is not the end.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Transmembrane domain of surface-exposed outer membrane lipoprotein RcsF is threaded through the lumen of β-barrel proteins.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Sensitization of microcin J25-resistant strains by a membrane-permeabilizing peptide.Applied and environmental microbiology · 2010Article
- Identification of TbpA residues required for transferrin-iron utilization by Neisseria gonorrhoeae.Infection and immunity · 2008Article
- Identification of transferrin-binding domains in TbpB expressed by Neisseria gonorrhoeae.Infection and immunity · 2007Article
- Mutational analysis of a bifunctional ferrisiderophore receptor and signal-transducing protein from Pseudomonas aeruginosa.Journal of bacteriology · 2005Article
- Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12.Journal of bacteriology · 2004Article
- Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coli.Journal of bacteriology · 2004Article
- Determination of surface-exposed, functional domains of gonococcal transferrin-binding protein A.Infection and immunity · 2004Article
- Crystal structure of the antibiotic albomycin in complex with the outer membrane transporter FhuA.Protein science : a publication of the Protein Society · 2000Article
- Prediction by a neural network of outer membrane beta-strand protein topology.Protein science : a publication of the Protein Society · 1998Article
- Colicin import into Escherichia coli cells.Journal of bacteriology · 1998Review
- Identification of a new site for ferrichrome transport by comparison of the FhuA proteins of Escherichia coli, Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans.Journal of bacteriology · 1998Article
- Specific in vivo labeling of cell surface-exposed protein loops: reactive cysteines in the predicted gating loop mark a ferrichrome binding site and a ligand-induced conformational change of the Escherichia coli FhuA protein.Journal of bacteriology · 1998Article
- Properties of the FhuA channel in the Escherichia coli outer membrane after deletion of FhuA portions within and outside the predicted gating loop.Journal of bacteriology · 1996Article
- Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.Journal of bacteriology · 1996Article
- Analysis of the Erwinia chrysanthemi ferrichrysobactin receptor gene: resemblance to the Escherichia coli fepA-fes bidirectional promoter region and homology with hydroxamate receptors.Journal of bacteriology · 1996Article
- Sequences of the Escherichia coli BtuB protein essential for its insertion and function in the outer membrane.Journal of bacteriology · 1995Article
- Topological analysis of the Escherichia coli ferrichrome-iron receptor by using monoclonal antibodies.Journal of bacteriology · 1995Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ferrichrome-iron receptor of Escherichia coli K-12 is FhuA (M(r), 78,992), the first component of an energy-dependent, high-affinity iron uptake pathway. FhuA is also the cognate receptor for bacteriophages T5, T1, phi 80, and UC-1, for colicin M and microcin 25, and for albomycin. To probe the topological organization of FhuA which enables recognition of these different ligands, we generated a library of 16 insertion mutations within the fhuA gene. Each insertion spliced a 13-amino-acid antigenic determinant (the C3 epitope of poliovirus) at a different position within FhuA. Immunoblotting of outer membranes with anti-FhuA and anti-C3 antibodies indicated that 15 of 16 FhuA.C3 proteins were present in the outer membrane in amounts similar to that observed for plasmid-encoded wild-type FhuA. One chimeric protein with the C3 epitope inserted after amino acid 440 of FhuA was present in the outer membrane in greatly reduced amounts. Strains overexpressing FhuA.C3 proteins were subjected to flow cytometric analysis using anti-FhuA monoclonal antibodies. Such analysis showed that (i) the chimeric proteins were properly localized and (ii) the wild-type FhuA protein structure had not been grossly altered by insertion of the C3 epitope. Twelve of sixteen strains expressing FhuA.C3 proteins were proficient in ferrichrome transport and remained sensitive to FhuA-specific phages. Three FhuA.C3 proteins, with insertions after amino acid 321, 405, or 417 of FhuA, were detected at the cell surface by flow cytometry using anti-C3 antibodies. These three chimeric proteins were all biologically active. We conclude that amino acids 321, 405, and 417 are surface accessible in wild-type FhuA.
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