ArticlePflugers Archiv : European journal of physiology1994
Effect of ATP, carbachol and other agonists on intracellular calcium activity and membrane voltage of pancreatic ducts.
Article in Pflugers Archiv : European journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 43 citations in OpenAlex.
- The cystic fibrosis of exocrine pancreas.Cold Spring Harbor perspectives in medicine · 2013Review
- Cell volume regulation in epithelial physiology and cancer.Frontiers in physiology · 2013Review
- Molecular mechanism of pancreatic and salivary gland fluid and HCO3 secretion.Physiological reviews · 2012Review
- Purinergic receptors in the endocrine and exocrine pancreas.Purinergic signalling · 2008Article
- P2Y2 and P2Y4 receptors regulate pancreatic Ca(2+)-activated K+ channels differently.Pflugers Archiv : European journal of physiology · 2005Article
- Luminal ATP stimulates fluid and HCO3- secretion in guinea-pig pancreatic duct.The Journal of physiology · 1999Article
- Interactions between secretin and acetylcholine in the regulation of fluid secretion by isolated rat pancreatic ducts.The Journal of physiology · 1996Article
- Calcium influx pathways in rat pancreatic ducts.Pflugers Archiv : European journal of physiology · 1996Article
- Flufenamate and Gd3+ inhibit stimulated Ca2+ influx in the epithelial cell line CFPAC-1.Pflugers Archiv : European journal of physiology · 1994Article
- Effect of secretin and inhibitors of HCO3-/H+ transport on the membrane voltage of rat pancreatic duct cells.Pflugers Archiv : European journal of physiology · 1993Article
- Molecular basis of potassium channels in pancreatic duct epithelial cells.Channels (Austin, Tex.)Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pancreatic duct has been regarded as a typical cAMP-regulated epithelium, and our knowledge about its Ca2+ homeostasis is limited. Hence, we studied the regulation of intracellular calcium, [Ca2+]i, in perfused rat pancreatic ducts using the Ca(2+)-sensitive probe fura-2. In some experiments we also measured the basolateral membrane voltage, Vbl, of individual cells. The resting basal [Ca2+]i was relatively high, corresponding to 263 +/- 28 nmol/l, and it decreased rapidly to 106 +/- 28 nmol/l after removal of Ca2+ from the bathing medium (n = 31). Carbachol increased [Ca2+]i in a concentration-dependent manner. At 10 mumol/l the fura-2 fluorescence ratio increased by 0.49 +/- 0.06 (n = 24), corresponding to an increase in [Ca2+]i by 111 +/- 15 nmol/l (n = 17). ATP, added to the basolateral side at 0.1 mmol/l and 1 mmol/l, increased the fluorescence ratio by 0.67 +/- 0.06 and 1.01 +/- 14 (n = 46; 12), corresponding to a [Ca2+]i increase of 136 +/- 22 nmol/l and 294 +/- 73 nmol/l respectively (n = 15; 10). Microelectrode measurements showed that ATP (0.1 mmol/l) hyperpolarized Vbl from -62 +/- 3 mV to -70 +/- 3 mV, an effect which was in some cases only transient (n = 7). This effect of ATP was different from that of carbachol, which depolarized Vbl. Applied together with secretin, ATP delayed the secretin-induced depolarization and prolonged the initial hyperpolarization of Vbl (n = 4). Several other putative agonists of pancreatic HCO3- secretion were also tested for their effects on [Ca2+]i.(ABSTRACT TRUNCATED AT 250 WORDS)
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