ArticleMolecular and cellular biology1994
RNA binding by Sxl proteins in vitro and in vivo.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 98 citations in OpenAlex.
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- A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates.Journal of visualized experiments : JoVE · 2018Article
- Concentration and Localization of Coexpressed ELAV/Hu Proteins Control Specificity of mRNA Processing.Molecular and cellular biology · 2015Article
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- Biochemical and functional analysis of Drosophila-sciara chimeric sex-lethal proteins.PloS one · 2013Article
- Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Molecular evolution of Drosophila Sex-lethal and related sex determining genes.BMC evolutionary biology · 2012Article
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- Forty years of the 93D puff of Drosophila melanogaster.Journal of biosciences · 2011Review
- The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster.PLoS genetics · 2011Article
- Pleiotropic consequences of misexpression of the developmentally active and stress-inducible non-coding hsrω gene in Drosophila.Journal of biosciences · 2011Article
- Quantitative approaches to monitor protein-nucleic acid interactions using fluorescent probes.RNA (New York, N.Y.) · 2011Review
- Path to facilitate the prediction of functional amino acid substitutions in red blood cell disorders--a computational approach.PloS one · 2011Article
- PPS, a large multidomain protein, functions with sex-lethal to regulate alternative splicing in Drosophila.PLoS genetics · 2010Article
- Functioning of the Drosophila Wilms'-tumor-1-associated protein homolog, Fl(2)d, in Sex-lethal-dependent alternative splicing.Genetics · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sxl has been proposed to regulate splicing of specific target genes by directly interacting with their pre-mRNAs. We have therefore examined the RNA-binding properties of Sxl protein in vitro and in vivo. Gel shift and UV cross-linking assays with a purified recombinant MBP-Sxl fusion protein demonstrated preferential binding to RNAs containing poly(U) tracts, and the protein footprinted over the poly(U) region. The protein did not appear to recognize either branch point or AG dinucleotide sequences, but an adenosine residue at the 5' end of the poly(U) tract enhanced binding severalfold. MBP-Sxl formed two shifted complexes on a tra regulated acceptor site RNA; the doubly shifted form may have been stabilized by protein-protein interactions. Consistent with its proposed role in pre-mRNA processing, in nuclear extracts Sxl was found in large ribonucleoprotein (RNP) complexes which sedimented significantly faster than bulk heterogeneous nuclear RNP and small nuclear RNPs. Anti-Sxl staining of polytene chromosomes showed Sxl protein at a number of chromosomal locations, among which was the Sxl locus itself. Sxl protein could also be targeted to a new chromosomal site carrying a transgene containing splicing regulatory sequences from the Sxl gene, following transcriptional induction. After prolonged heat shock, all Sxl protein was restricted to the heat-induced puff at the hs93D locus. In contrast, a presumptive small nuclear RNP protein was observed at several heat puffs following shock.
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