ArticleMolecular and cellular biology1994
The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Biallelic variants in SNUPN cause a limb girdle muscular dystrophy with myofibrillar-like features.Brain : a journal of neurology · 2024Article
- Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family.Scientific reports · 2021Article
- BRCA1 binds TERRA RNA and suppresses R-Loop-based telomeric DNA damage.Nature communications · 2021Article
- Targeted Quantification of Detergent-Insoluble RNA-Binding Proteins in Human Brain Reveals Stage and Disease Specific Co-aggregation in Alzheimer's Disease.Frontiers in molecular neuroscience · 2021Article
- Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6.Nucleic acids research · 2019Article
- RNA-binding proteins with basic-acidic dipeptide (BAD) domains self-assemble and aggregate in Alzheimer's disease.The Journal of biological chemistry · 2018Article
- Cajal bodies and snRNPs - friends with benefits.RNA biology · 2017Review
- U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish.Nucleic acids research · 2015Article
- Review
- Analyses of in vivo interaction and mobility of two spliceosomal proteins using FRAP and BiFC.PloS one · 2008Article
- Article
- prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast.Nature structural & molecular biology · 2007Article
- Escaping the nuclear confines: signal-dependent pre-mRNA splicing in anucleate platelets.Cell · 2005Article
- Association of polyadenylation cleavage factor I with U1 snRNP.RNA (New York, N.Y.) · 2003Article
- A conserved Drosophila transportin-serine/arginine-rich (SR) protein permits nuclear import of Drosophila SR protein splicing factors and their antagonist repressor splicing factor 1.Molecular biology of the cell · 2002Article
- The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins.The Plant cell · 1998Article
- Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.Molecular and cellular biology · 1997Article
- Structure and expression of a plant U1 snRNP 70K gene: alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts.The Plant cell · 1996Article
- Nuclear localization signals overlap DNA- or RNA-binding domains in nucleic acid-binding proteins.Nucleic acids research · 1995Review
- Splinkerettes--improved vectorettes for greater efficiency in PCR walking.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Expression of the recombinant human U1-70K protein in COS cells resulted in its rapid transport to the nucleus, even when binding to U1 RNA was debilitated. Deletion analysis of the U1-70K protein revealed the existence of two segments of the protein which were independently capable of nuclear localization. One nuclear localization signal (NLS) was mapped within the U1 RNA-binding domain and consists of two typically separated but interdependent elements. The major element of this NLS resides in structural loop 5 between the beta 4 strand and the alpha 2 helix of the folded RNA recognition motif. The C-terminal half of the U1-70K protein which was capable of nuclear entry contains two arginine-rich regions, which suggests the existence of a second NLS. Site-directed mutagenesis of the RNA recognition motif NLS demonstrated that the U1-70K protein can be transported independently of U1 RNA and that its association with the U1 small nuclear ribonucleoprotein particle can occur in the nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.