ArticleNaunyn-Schmiedeberg's archives of pharmacology1994
The isoquinoline derivative LOE 908 selectively blocks vasopressin-activated nonselective cation currents in A7r5 aortic smooth muscle cells.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 58 citations in OpenAlex.
- Long-term maternal hypoxia: the role of extracellular Ca2+ entry during serotonin-mediated contractility in fetal ovine pulmonary arteries.Reproductive sciences (Thousand Oaks, Calif.) · 2011Article
- Preservation of serotonin-mediated contractility in adult sheep pulmonary arteries following long-term high-altitude hypoxia.High altitude medicine & biology · 2011Article
- Protein kinase Ctheta negatively regulates store-independent Ca2+ entry and phosphatidylserine exposure downstream of glycoprotein VI in platelets.The Journal of biological chemistry · 2010Article
- Stimulated calcium entry and constitutive RhoA kinase activity cause stretch-induced detrusor contraction.European journal of pharmacology · 2008Article
- Non-selective cation channels, transient receptor potential channels and ischemic stroke.Biochimica et biophysica acta · 2007Review
- Vasopressin stimulates action potential firing by protein kinase C-dependent inhibition of KCNQ5 in A7r5 rat aortic smooth muscle cells.American journal of physiology. Heart and circulatory physiology · 2007Article
- Regulation of TRPM2 channels in neutrophil granulocytes by ADP-ribose: a promising pharmacological target.Naunyn-Schmiedeberg's archives of pharmacology · 2005Review
- Pharmacological profile of store-operated channels in cerebral arteriolar smooth muscle cells.British journal of pharmacology · 2003Article
- The developing relationship between receptor-operated and store-operated calcium channels in smooth muscle.British journal of pharmacology · 2002Review
- Dependency of detrusor contractions on calcium sensitization and calcium entry through LOE-908-sensitive channels.British journal of pharmacology · 2001Article
- Troglitazone and pioglitazone attenuate agonist-dependent Ca2+ mobilization and cell proliferation in vascular smooth muscle cells.British journal of pharmacology · 1999Article
- A non-capacitative pathway activated by arachidonic acid is the major Ca2+ entry mechanism in rat A7r5 smooth muscle cells stimulated with low concentrations of vasopressin.The Journal of physiology · 1999Article
- Activation of three types of voltage-independent Ca2+ channel in A7r5 cells by endothelin-1 as revealed by a novel Ca2+ channel blocker LOE 908.British journal of pharmacology · 1999Article
- Monovalent cation and L-type Ca2+ channels participate in calcium paradox-like phenomenon in rabbit aortic smooth muscle cells.The Journal of physiology · 1999Article
- Effects of extracellular pH on receptor-mediated Ca2+ influx in A7r5 rat smooth muscle cells: involvement of two different types of channel.The Journal of physiology · 1997Article
- Inhibition of a store-operated Ca2+ entry pathway in human endothelial cells by the isoquinoline derivative LOE 908.British journal of pharmacology · 1996Article
- Investigations on the mechanism of action of the antiproliferant and ion channel antagonist flufenamic acid.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The effect of (R,S)-(3,4-dihydro 6,7-dimethoxy-isoquinoline-1-yl)-2-phenyl- N,N-di-[2-(2,3,4-trimethoxyphenyl)ethyl]-acetamide (LOE 908), a cation channel blocker in HL-60 promyeloblasts, was studied in the A7r5 smooth muscle cell line from rat thoracic aorta, using the whole-cell patch-clamp technique. At a holding potential of -60 mV, application of vasopressin induced a nonselective cation conductance in voltage-clamped A7r5 cells. The current-voltage relation was linear, and currents reversed close to 0 mV regardless of the chloride gradient. The activation of the nonselective cation conductance by vasopressin was not affected by dialysing cells with Ca(2+)-free internal solution. LOE 908 blocked this current in a concentration-dependent manner with an IC50 of 560 nM, whereas dihydropyridine-sensitive Ba2+ current through voltage-dependent Ca2+ channels was blocked with an IC50 of 28 microM. Another organic blocker of receptor-mediated Ca2+ entry, 1-beta-[3-(4-methoxyphenyl)-propoxy]-4-methoxyphenethyl-1H-imidazole hydrochloride (SK&F 96365), blocked both, the vasopressin-induced nonselective conductance and the voltage-activated Ba2+ current with similar IC50 values of 13 microM and 8 microM, respectively. The rank order of potency of inorganic blockers on the vasopressin-induced inward current was Gd3+ > La3+ > Cd2+. Vasopressin-induced non-selective cation current was also observed in pertussis toxin-pretreated A7r5 cells but was completely abolished after infusion of the GDP analogue, guanosine 5'-O-[3-thio]diphosphate, from the patch pipette. Furthermore, vasopressin induced a transient outward current, suggesting a Ca(2+)-activated K(+)-current, which overlapped with the nonselective cation conductance.(ABSTRACT TRUNCATED AT 250 WORDS)
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