ArticleMolecular and cellular biology1994
Preferential deadenylation of Hsp70 mRNA plays a key role in regulating Hsp70 expression in Drosophila melanogaster.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- The Dynamics of Cytoplasmic mRNA Metabolism.Molecular cell · 2020Article
- Heat Shock Responsive Gene Expression Modulated by mRNA Poly(A) Tail Length.Frontiers in plant science · 2020Article
- lncRNAs in Stress Response.Current topics in microbiology and immunology · 2016Review
- Non-coding RNAs turn up the heat: an emerging layer of novel regulators in the mammalian heat shock response.Cell stress & chaperones · 2014Review
- Robust heat-inducible gene expression by two endogenous hsp70-derived promoters in transgenic Aedes aegypti.Insect molecular biology · 2012Article
- Regulation of survival gene hsp70.Cell stress & chaperones · 2012Review
- MicroRNAs in cardiac disease.Translational research : the journal of laboratory and clinical medicine · 2011Review
- Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation.RNA (New York, N.Y.) · 2010Article
- The 2008 Genetics Society of America Medal. Susan Lindquist.Genetics · 2008Article
- Translation-independent inhibition of mRNA deadenylation during stress in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2006Article
- Reduced stress tolerance of glutamine-deprived human monocytic cells is associated with selective down-regulation of Hsp70 by decreased mRNA stability.Journal of molecular medicine (Berlin, Germany) · 2006Article
- A complex containing the CCR4 and CAF1 proteins is involved in mRNA deadenylation in Drosophila.The EMBO journal · 2004Article
- Regulation of heat shock proteins, Hsp70 and Hsp64, in heat-shocked Malpighian tubules of Drosophila melanogaster larvae.Cell stress & chaperones · 2002Article
- Messenger RNA-binding properties of nonpolysomal ribonucleoproteins from heat-stressed tomato cellsPlant physiology · 1999Article
- The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes.The EMBO journal · 1998Article
- Differential display-mediated isolation of a genomic sequence for a putative mitochondrial LMW HSP specifically expressed in condition of induced thermotolerance in Arabidopsis thaliana (L.) heynh.Plant molecular biology · 1997Article
- Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR.Applied and environmental microbiology · 1996Article
- HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences.Chromosoma · 1996Article
- mRNA stability in mammalian cells.Microbiological reviews · 1995Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Following a standard heat shock, approximately 40% of Hsp70 transcripts in Drosophila melanogaster lack a poly(A) tail. Since heat shock disrupts other aspects of RNA processing, this observation suggested that heat might disrupt polyadenylation as well. We find, however, that as the temperature is increased a larger fraction of Hsp70 RNA is polyadenylated. Poly(A)-deficient Hsp70 RNAs arise not from a failure in polyadenylation but from the rapid and selective removal of poly(A) from previously adenylated transcripts. Poly(A) removal is highly regulated: poly(A) is (i) removed much more rapidly from Hsp70 RNAs than from Hsp23 RNAs, (ii) removed more rapidly after mild heat shocks than after severe heat shocks, and (iii) removed more rapidly after a severe heat shock if cells have first been conditioned by a mild heat treatment. Poly(A) seems to be removed by simple deadenylation rather than by endonucleolytic cleavage 5' of the adenylation site. During recovery from heat shock, deadenylation is rapidly followed by degradation. In cells maintained at high temperatures, however, the two processes are uncoupled and Hsp70 RNAs are deadenylated without being degraded. These deadenylated mRNAs are translated with low efficiency. Deadenylation therefore allows Hsp70 synthesis to be repressed even when degradation of the mRNA is blocked. Poly(A) tail shortening appears to play a key role in regulating Hsp70 expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.