ArticleThe EMBO journal1994
Reverse transcriptase activity of an intron encoded polypeptide.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 51 citations in OpenAlex.
- A Network of Pathways Controlling Cellular Homeostasis Affects the Onset of Senescence inJournal of fungi (Basel, Switzerland) · 2021Review
- Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Function of chloroplast RNA-binding proteins.Cellular and molecular life sciences : CMLS · 2011Review
- The complete chloroplast genome sequence of the chlorophycean green alga Scenedesmus obliquus reveals a compact gene organization and a biased distribution of genes on the two DNA strands.BMC evolutionary biology · 2006Article
- Sensitivity to Alternaria alternata toxin in citrus because of altered mitochondrial RNA processing.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Mitochondrial DNA rearrangements of Podospora anserina are under the control of the nuclear gene grisea.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Contribution of ultra-short invasive elements to the evolution of the mitochondrial genome in the genus Podospora.Nucleic acids research · 1996Article
- Structural and functional analysis of plastid genomes from parasitic plants: loss of an intron within the genus Cuscuta.Current genetics · 1995Article
- RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.).Nucleic acids research · 1995Article
- Homing of a group II intron in yeast mitochondrial DNA is accompanied by unidirectional co-conversion of upstream-located markers.The EMBO journal · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of group II introns from eukaryotic organelles and prokaryotes contain open reading frames for polypeptides with homology to retroviral reverse transcriptases (RTs). We have used the yeast transposon (Ty) system to express ORFs for RTs from eukaryotic organelles. This includes the mitochondrial coxI intron i1 from the fungus Podospora anserina, the plastid petD intron from the alga Scenedesmus obliquus and the mitochondrial RTL gene from the alga Chlamydomonas reinhardtii. The ORFs were fused with the TYA ORF from the yeast retrotransposon Ty to produce virus-like particles in the recipient strains with detectable amounts of the RT-like polypeptides. Analysis of the heterologous gene products revealed biochemical evidence that the P. anserina intron encodes an RNA-directed DNA polymerase with properties typically found for RTs of viral or retrotransposable origin. In vitro assays showed that the intron encoded RT is sensitive to RT inhibitors such as N-ethylmaleimide and dideoxythymidine triphosphate but is insensitive against the DNA polymerase inhibitor aphidicolin. The direct biochemical evidence provided here supports the idea that intron encoded RTs are involved in intron transposition events.
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