ArticleProceedings of the National Academy of Sciences of the United States of America1994
Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 66 citations in OpenAlex.
- Cryo-electron microscopy visualization of a large insertion in the 5S ribosomal RNA of the extremely halophilic archaeon Halococcus morrhuae.FEBS open bio · 2020Article
- Characterization of conserved bases in 4.5S RNA of Escherichia coli by construction of new F' factors.Journal of bacteriology · 2008Article
- Changes in the conformation of 5S rRNA cause alterations in principal functions of the ribosomal nanomachine.Nucleic acids research · 2007Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- The three-dimensional architecture of the class I ligase ribozyme.RNA (New York, N.Y.) · 2004Article
- Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.RNA (New York, N.Y.) · 2002Article
- Mining biochemical information: lessons taught by the ribosome.RNA (New York, N.Y.) · 2002Article
- Saturation mutagenesis of 5S rRNA in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway.The Journal of cell biology · 1999Article
- The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases.The EMBO journal · 1998Article
- Structure of 5S rRNA within the Escherichia coli ribosome: iodine-induced cleavage patterns of phosphorothioate derivatives.RNA (New York, N.Y.) · 1998Article
- New features of 23S ribosomal RNA folding: the long helix 41-42 makes a "U-turn" inside the ribosome.RNA (New York, N.Y.) · 1998Article
- The environment of 5S rRNA in the ribosome: cross-links to the GTPase-associated area of 23S rRNA.Nucleic acids research · 1998Article
- A new technique for the characterization of long-range tertiary contacts in large RNA molecules: insertion of a photolabel at a selected position in 16S rRNA within the Escherichia coli ribosome.Nucleic acids research · 1997Article
- Molecular genetics of the peptidyl transferase center and the unusual Var1 protein in yeast mitochondrial ribosomes.Experientia · 1996Review
- Loop IV of 5S ribosomal RNA has contacts both to domain II and to domain V of the 23S RNA.RNA (New York, N.Y.) · 1996Article
- The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site.Nucleic acids research · 1995Article
- Insights into erythromycin action from studies of its activity as inducer of resistance.Antimicrobial agents and chemotherapy · 1995Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A DNA fragment containing the Escherichia coli 5S rDNA sequence linked to a T7 promoter was prepared by PCR from an M13 clone carrying the 5S-complementary sequence. The DNA was transcribed with T7 polymerase using a mixture of [alpha-32P]UTP and 4-thio-UTP, yielding a transcript in which approximately 18% of the uridine residues were randomly replaced by thiouridine. This modified 5S RNA could be reconstituted efficiently into 50S ribosomal subunits or 70S functional complexes. The reconstituted particles were irradiated at wavelengths above 300 nm, and the crosslinked ribosomal components were identified. A crosslink in high yield was reproducibly observed between the modified 5S RNA and 23S RNA, involving residue U-89 of the 5S RNA (at the loop end of helix IV) linked to nucleotide 2477 of the 23S RNA in the loop end of helix 89, immediately adjacent to the peptidyltransferase "ring." On the basis of this result, and in combination with earlier immunoelectron microscopic data, we propose a model for the orientation of the 5S RNA in the 50S subunit.
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