ArticleThe Journal of cell biology1994
Staurosporine inhibits agrin-induced acetylcholine receptor phosphorylation and aggregation.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 102 citations in OpenAlex.
- Phosphorylation of Sar1b protein releases liver fatty acid-binding protein from multiprotein complex in intestinal cytosol enabling it to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron transport vesicle.The Journal of biological chemistry · 2012Article
- Cholesterol and lipid microdomains stabilize the postsynapse at the neuromuscular junction.The EMBO journal · 2006Article
- Induction of myasthenia by immunization against muscle-specific kinase.The Journal of clinical investigation · 2006Article
- Neuregulin induces the expression of transcription factors and myosin heavy chains typical of muscle spindles in cultured human muscle.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Laminin-1 redistributes postsynaptic proteins and requires rapsyn, tyrosine phosphorylation, and Src and Fyn to stably cluster acetylcholine receptors.The Journal of cell biology · 2002Article
- Clustering of nicotinic acetylcholine receptors: from the neuromuscular junction to interneuronal synapses.Molecular neurobiology · 2002Review
- Src-class kinases act within the agrin/MuSK pathway to regulate acetylcholine receptor phosphorylation, cytoskeletal anchoring, and clustering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Src, Fyn, and Yes are not required for neuromuscular synapse formation but are necessary for stabilization of agrin-induced clusters of acetylcholine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Agrin-induced phosphorylation of the acetylcholine receptor regulates cytoskeletal anchoring and clustering.The Journal of cell biology · 2001Article
- Agrin controls synaptic differentiation in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- The myristoylated protein rapsyn is cotargeted with the nicotinic acetylcholine receptor to the postsynaptic membrane via the exocytic pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Evidence of an agrin receptor in cortical neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Specific agrin isoforms induce cAMP response element binding protein phosphorylation in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Laminin-induced acetylcholine receptor clustering: an alternative pathway.The Journal of cell biology · 1997Article
- Association of muscle-specific kinase MuSK with the acetylcholine receptor in mammalian muscle.The EMBO journal · 1997Article
- Kinase domain of the muscle-specific receptor tyrosine kinase (MuSK) is sufficient for phosphorylation but not clustering of acetylcholine receptors: required role for the MuSK ectodomain?Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Tyrosine phosphorylation of nicotinic acetylcholine receptor mediates Grb2 binding.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Intercellular communication that mediates formation of the neuromuscular junction.Molecular neurobiology · 1997Review
- Common molecular mechanisms in field- and agrin-induced acetylcholine receptor clustering.Cellular and molecular neurobiology · 1997Article
- Laminin-induced clustering of dystroglycan on embryonic muscle cells: comparison with agrin-induced clustering.The Journal of cell biology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Agrin, a protein that mediates nerve-induced acetylcholine receptor (AChR) aggregation at developing neuromuscular junctions, has been shown to cause an increase in phosphorylation of the beta, gamma, and delta subunits of AChRs in cultured myotubes. As a step toward understanding the mechanism of agrin-induced AChR aggregation, we examined the effects of inhibitors of protein kinases on AChR aggregation and phosphorylation in chick myotubes in culture. Staurosporine, an antagonist of both protein serine and tyrosine kinases, blocked agrin-induced AChR aggregation in a dose-dependent manner; 50% inhibition occurred at approximately 2 nM. The extent of inhibition was independent of agrin concentration, suggesting an effect downstream of the interaction of agrin with its receptor. Staurosporine blocked agrin-induced phosphorylation of the AChR beta subunit, which occurs at least in part on tyrosine residues, but did not reduce phosphorylation of the gamma and delta subunits, which occurs on serine/threonine residues. Staurosporine also prevented the agrin-induced decrease in the rate at which AChRs are extracted from intact myotubes by mild detergents. H-7, an antagonist of protein serine kinases, inhibited agrin-induced phosphorylation of the gamma and delta subunits but did not block agrin-induced phosphorylation of the AChR beta subunit, AChR aggregation, or the decrease in AChR extractability. The results provide support for the hypothesis that tyrosine phosphorylation of the beta subunit plays a role in agrin-induced AChR aggregation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.