ArticleThe Journal of clinical investigation1994
Bacterial lipopolysaccharide primes human neutrophils for enhanced release of arachidonic acid and causes phosphorylation of an 85-kD cytosolic phospholipase A2.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 107 citations in OpenAlex.
- Amelioration of experimentally induced inflammatory arthritis by intra-articular injection of visnagin.Current research in pharmacology and drug discovery · 2022Article
- Essential Role for Ethanolamine Plasmalogen Hydrolysis in Bacterial Lipopolysaccharide Priming of Macrophages for Enhanced Arachidonic Acid Release.Frontiers in immunology · 2017Article
- Multiple Phenotypic Changes Define Neutrophil Priming.Frontiers in cellular and infection microbiology · 2017Review
- LEUKOCYTE LIPID BODIES - STRUCTURE AND FUNCTION AS "EICOSASOMES".Transactions of the American Clinical and Climatological Association · 2016Article
- Janus kinase 3 activity is necessary for phosphorylation of cytosolic phospholipase A2 and prostaglandin E2 synthesis by macrophages infected with Francisella tularensis live vaccine strain.Infection and immunity · 2014Article
- Regulation of arachidonate remodeling enzymes impacts eosinophil survival during allergic asthma.American journal of respiratory cell and molecular biology · 2009Article
- Inhibition of LPS-induced chemokine production in human lung endothelial cells by lipid conjugates anchored to the membrane.British journal of pharmacology · 2002Article
- Epstein-Barr virus primes human polymorphonuclear leucocytes for the biosynthesis of leukotriene B4.Clinical and experimental immunology · 2001Article
- Role of p38 in the priming of human neutrophils by peritoneal dialysis effluent.Clinical and diagnostic laboratory immunology · 1999Article
- Activation of phospholipase A2 in human neutrophils by polyunsaturated fatty acids and its role in stimulation of superoxide production.The Biochemical journal · 1998Article
- Role of arachidonic acid and its metabolites in the priming of NADPH oxidase in human polymorphonuclear leukocytes by peritoneal dialysis effluent.Clinical and diagnostic laboratory immunology · 1998Article
- Co-compartmentalization of MAP kinases and cytosolic phospholipase A2 at cytoplasmic arachidonate-rich lipid bodies.The American journal of pathology · 1998Article
- Neutrophils exposed to bacterial lipopolysaccharide upregulate NADPH oxidase assembly.The Journal of clinical investigation · 1998Article
- Activation of neutral sphingomyelinase in human neutrophils by polyunsaturated fatty acids.Immunology · 1997Article
- Studies of skin-window exudate human neutrophils: increased resistance to pentoxifylline of the respiratory burst in primed cells.Inflammation · 1997Article
- Induction of cytosolic phospholipase A2 activity by phosphatidic acid and diglycerides in permeabilized human neutrophils: interrelationship between phospholipases D and A2.The Biochemical journal · 1997Article
- Potent CD14-mediated signalling of human leukocytes by Escherichia coli can be mediated by interaction of whole bacteria and host cells without extensive prior release of endotoxin.Infection and immunity · 1996Article
- Determinants of activation by complement of group II phospholipase A2 acting against Escherichia coli.Infection and immunity · 1996Article
- Cyclooxygenase-2 and its regulation in inflammation.Mediators of inflammation · 1996Article
- Cell surface-bound elastase and cathepsin G on human neutrophils: a novel, non-oxidative mechanism by which neutrophils focus and preserve catalytic activity of serine proteinases.The Journal of cell biology · 1995Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of leukotriene B4 (LTB4) by human neutrophils (PMN) in response to different stimuli is increased after pretreatment with lipopolysaccharides (LPS). We have analyzed the steps in arachidonic acid (AA) metabolism affected by LPS by examining release of AA and its metabolites from [3H]AA prelabeled PMN. Pretreatment of PMN for 60 min with up to 1 microgram/ml of LPS alone had no effect, but release of [3H]AA was stimulated up to fivefold during subsequent stimulation with a second agent. In the absence of LPS-binding protein (LBP), priming was maximal after pretreatment of PMN with 10 ng of LPS/ml for 60 min; in the presence of LBP maximal priming occurred within 45 min at 0.1 ng of LPS/ml and within 15 min at 100 ng of LPS/ml. Treatment of PMN with 10 ng of LPS/ml also increased uptake of opsonized zymosan by up to 60%. Phospholipids are the source of released [3H]AA. No release was observed from [14C]oleic acid (OA)-labeled PMN suggesting that phospholipolysis may be specific for [3H]AA-labeled phospholipid pools. Cytosol from PMN primed with LPS contains two to three times the phospholipase A2 (PLA2) activity of control PMN, against 1-palmitoyl-[2-14C]arachidonoyl-phosphatidylcholine. This activity is Ca2+ dependent and dithiothreitol resistant. LPS priming is accompanied by reduced migration during SDS-PAGE of an 85-kD protein, identified as a cytosolic PLA2. The extent and kinetics of this effect of LPS on cPLA2 parallel the priming of [3H]AA release, both depending on LPS concentration either with or without LBP. These findings suggest that priming by LPS of AA metabolism by PMN includes phosphorylation of an AA-phospholipid-selective cytosolic PLA2 that is dissociated from activation until a second stimulus is applied.
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