ArticleNucleic acids research1994
A group III twintron encoding a maturase-like gene excises through lariat intermediates.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Characterization of the mitochondrial genomes forIMA fungus · 2025Article
- Unprecedented frequency of mitochondrial introns in colonial bilaterians.Scientific reports · 2022Article
- A functional twintron, 'zombie' twintrons and a hypermobile group II intron invading itself in plant mitochondria.Nucleic acids research · 2020Article
- Chloroplast genome expansion by intron multiplication in the basal psychrophilic euglenoidPeerJ · 2017Article
- Analysis of the Mitochondrial Genome in Hypomyces aurantius Reveals a Novel Twintron Complex in Fungi.International journal of molecular sciences · 2016Article
- Review
- Group II introns in eubacteria and archaea: ORF-less introns and new varieties.RNA (New York, N.Y.) · 2008Article
- Recent horizontal intron transfer to a chloroplast genome.Nucleic acids research · 2004Article
- The DIVa maturase binding site in the yeast group II intron aI2 is essential for intron homing but not for in vivo splicing.Molecular and cellular biology · 2003Article
- Putative proteins related to group II intron reverse transcriptase/maturases are encoded by nuclear genes in higher plants.Nucleic acids research · 2003Article
- Lariat formation and a hydrolytic pathway in plant chloroplast group II intron splicing.The EMBO journal · 2002Article
- Mechanism of maturase-promoted group II intron splicing.The EMBO journal · 2001Article
- Precise branch point mapping and quantification of splicing intermediates.Nucleic acids research · 1997Article
- Evidence for the late origin of introns in chloroplast genes from an evolutionary analysis of the genus Euglena.Nucleic acids research · 1995Article
- Two group I ribozymes with different functions in a nuclear rDNA intron.The EMBO journal · 1995Article
- Characterization of the unique intron-exon junctions of Euglena gene(s) encoding the polyprotein precursor to the light-harvesting chlorophyll a/b binding protein of photosystem II.Nucleic acids research · 1994Article
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5 authors.
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Abstract
The 1605 bp intron 4 of the Euglena gracilis chloroplast psbC gene was characterized as a group III twintron composed of an internal 1503 nt group III intron with an open reading frame of 1374 nt (ycf13, 458 amino acids), and an external group III intron of 102 nt. Twintron excision proceeds by a sequential splicing pathway. The splicing of the internal and external group III introns occurs via lariat intermediates. Branch sites were mapped by primer extension RNA sequencing. The unpaired adenosines in domains VI of the internal and external introns are covalently linked to the 5' nucleotide of the intron via 2'-5' phosphodiester bonds. This bond is susceptible to hydrolysis by the debranching activity of the HeLa nuclear S100 fraction. The internal intron and presumptive ycf13 mRNA accumulates primarily as a linear RNA, although a lariat precursor can also be detected. The ycf13 gene encodes a maturase-like protein that may be involved in group III intron metabolism.
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