ArticleBiophysical journal1994
Correction for missed events based on a realistic model of a detector.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter.FEBS letters · 2017Article
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.The Journal of membrane biology · 2006Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
- A subsequent fit of time series and amplitude histogram of patch-clamp records reveals rate constants up to 1 per microsecond.The Journal of membrane biology · 2005Article
- Distributions-per-level: a means of testing level detectors and models of patch-clamp data.The Journal of membrane biology · 2004Article
- The anomalous mole fraction effect in Chara: gating at the edge of temporal resolution.Biophysical journal · 2000Article
- Fluctuation analysis of motor protein movement and single enzyme kinetics.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Estimation of kinetic rate constants from multi-channel recordings by a direct fit of the time series.Biophysical journal · 1994Article
- Detection of jumps in single-channel data containing subconductance levels.Biophysical journal · 1994Article
- Fast single-channel measurements resolve the blocking effect of Cs+ on the K+ channel.Biophysical journal · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative patch-clamp analysis based on dwell-time histograms has to deal with the problem of missed events. The correction of the evaluated time constants has to take into account the characteristics of the detector used for the reconstruction of the time series. In previous approaches a simple model of the detector has been used, which is based on the assumption that all events shorter than the temporal resolution tres were missed, irrespective of the preceding events. Rather than the standard assumption of a fixed dead time, we introduce a more realistic model of a detector by a continuous-time version of the Hinkley detector. The combined state of the channel and the detector obeys a Markov model, which is governed by a Fokker-Planck-Kolmogorov partial differential equation. The steady-state solution leads to the determination of the apparent time constants tau o and tau c depending on the true rate constants koc and kco and the temporal resolution tres of the detector. Simulations with different kinds of detectors, including the Bessel filter with half-amplitude threshold detection, are performed. They show that our new equation predicts the dependence of tau c and tau o on koc, kco, and tres better than the standard equation used until now.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.