ArticleProceedings of the National Academy of Sciences of the United States of America1994
K+ currents expressed from the guinea pig cardiac IsK protein are enhanced by activators of protein kinase C.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- The focal adhesion protein Testin modulates KCNE2 potassium channel β subunit activity.Channels (Austin, Tex.) · 2021Article
- Control of Biophysical and Pharmacological Properties of Potassium Channels by Ancillary Subunits.Handbook of experimental pharmacology · 2021Article
- The auxiliary subunit KCNE1 regulates KCNQ1 channel response to sustained calcium-dependent PKC activation.PloS one · 2020Article
- Review
- KCNE Regulation of K(+) Channel Trafficking - a Sisyphean Task?Frontiers in physiology · 2012Article
- Protein kinase C downregulates I(Ks) by stimulating KCNQ1-KCNE1 potassium channel endocytosis.Heart rhythm · 2011Article
- O-glycosylation of the cardiac I(Ks) complex.The Journal of physiology · 2011Article
- MinK-dependent internalization of the IKs potassium channel.Cardiovascular research · 2009Article
- Simulation of developmental changes in action potentials with ventricular cell models.Systems and synthetic biology · 2007Article
- Cross-talk between beta(1)-adrenoceptors and ET(A) receptors in modulation of the slow component of delayed rectifier K(+) currents.Naunyn-Schmiedeberg's archives of pharmacology · 2005Article
- Properties, expression and potential roles of cardiac K+ channel accessory subunits: MinK, MiRPs, KChIP, and KChAP.The Journal of membrane biology · 2003Review
- Adrenergic control of the ultrarapid delayed rectifier current in canine atrial myocytes.The Journal of physiology · 1999Article
- I(sK) Channel in Strial Marginal Cells. Voltage-Dependence, Ion-Selectivity, Inhibition by 293B and Sensitivity to Clofilium.Auditory neuroscience · 1997Article
- Inhibition of IKs in guinea pig cardiac myocytes and guinea pig IsK channels by the chromanol 293B.Pflugers Archiv : European journal of physiology · 1996Article
- Characterization of a beta-adrenergically inhibited K+ current in rat cardiac ventricular cells.The Journal of physiology · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated cardiac cDNA and genomic clones encoding the guinea pig IsK protein. The deduced amino acid sequence is approximately 78% identical to the rat, mouse, and human variants of this channel, and the structure of the gene encoding the protein is also similar to that in other species. For example, the gene is present only once in the haploid genome, the protein-coding sequence is present on a single uninterrupted exon, an intron exists in the 5' untranslated domain, and multiple alternative polyadenylation sites are used in processing the transcript. Expression of the guinea pig protein in Xenopus oocytes results in a slowly activating, voltage-dependent K+ current, IsK, similar to those expressed previously from the rat, mouse, and human genes. However, in sharp contrast to the rat and mouse currents, activation of protein kinase C with phorbol esters increases the amplitude of the guinea pig IsK current, analogous to its effects on the endogenous IKs current in guinea pig cardiac myocytes. Mutagenesis of the guinea pig cDNA to alter four cytoplasmic amino acid residues alters the phenotype of the current response to protein kinase C from enhancement to inhibition, mimicking that of rat and mouse IsK currents. This mutation is consistent with reports that phosphorylation of Ser-102 by protein kinase C decreases the current amplitude. These data explain previously reported differences in the regulatory properties between recombinant rat or mouse IsK channels and native guinea pig IKs channels and provide further evidence that the IsK protein forms the channels that underlie the IKs current in the heart.
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