ArticleNucleic acids research1994
The existence of eukaryotic ribonucleoprotein consensus sequence-type RNA-binding proteins in a prokaryote, Synechococcus 6301.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 58 citations in OpenAlex.
- The RRM domain-containing protein Rbp3 interacts with ribosomes and the 3' ends of mRNAs encoding photosynthesis proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.RNA biology · 2024Article
- Roles for theJournal of biological rhythms · 2023Article
- Article
- Identification of genes expressed by Cryptococcus gattii during iron deprivation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation.Molecular genetics and genomics : MGG · 2006Article
- Molecular and biochemical characterization of a protein cyclophilin from the nematode Haemonchus contortus( P ).Parasitology research · 2005Article
- Chloroplast RNA-binding proteins.Current genetics · 2003Review
- Proteins released by Helicobacter pylori in vitro.Journal of bacteriology · 2002Article
- A family of RRM-type RNA-binding proteins specific to plant mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Involvement of the 5'-untranslated region in cold-regulated expression of the rbpA1 gene in the cyanobacterium Anabaena variabilis M3.Nucleic acids research · 1998Article
- A period-extender gene, pex, that extends the period of the circadian clock in the cyanobacterium Synechococcus sp. strain PCC 7942.Journal of bacteriology · 1998Article
- Regulation of gene expression in chloroplasts of higher plants.Plant molecular biology · 1996Review
- Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells.Plant molecular biology · 1996Article
- Physical and gene maps of the unicellular cyanobacterium Synechococcus sp. strain PCC6301 genome.Plant molecular biology · 1996Article
- Functional characteristics of the maize RNA-binding protein MA16.Plant molecular biology · 1995Article
- A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3.Nucleic acids research · 1995Article
- Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1.Molecular & general genetics : MGG · 1995Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A group of proteins containing a conserved ribonucleoprotein consensus sequence (RNP-CS)-type RNA-binding domain (CS-RBD) of approximately 80 amino acids is present in eukaryotic cells and binds specifically to a wide variety of RNA molecules. We have isolated 12 kDa single-stranded DNA binding proteins from the unicellular cyanobacterium Synechococcus 6301. The amino-terminal sequence was determined and two distinct genomic clones were isolated from a Synechococcus 6301 genomic library. Sequence analysis revealed that two closely related proteins contain a single CS-RBD of 82 amino acids and are named as 12RNP1 and 12RNP2. Both of the CS-RBDs share the highest amino acid identity with those of chloroplast ribonucleoproteins (40-51%). The 12RNP proteins were expressed in Escherichia coli bearing plasmids encoding glutathione S-transferase/12RNP fusion proteins and subjected to in vitro nucleic acid-binding assay. Both 12RNP1 and 12RNP2 bind to RNA homopolymers poly(U) and poly(G), indicating that they might be RNA-binding proteins. This is the first example of such proteins in prokaryotes. The 12RNP1 and 12RNP2 genes are transcribed as monocistronic mRNAs and the steady-state mRNA level of 12RNP1 is over 20-fold than that of 12RNP2. Due to the easiness of genetic manipulations the cyanobacterium will provide an excellent system to analyze the function of not only cyanobacterial but also plant RNA-binding proteins.
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