ArticleJournal of bacteriology1994
The femC locus of Staphylococcus aureus required for methicillin resistance includes the glutamine synthetase operon.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 108 citations in OpenAlex.
- A c-di-AMP-controlled glutamine synthesis pathway promotes persistence ofbioRxiv : the preprint server for biology · 2026Article
- Integrated multi-omics reveals coordinatedmSystems · 2026Article
- RefSeq-corrected transcriptomic and RNA-interaction integration maps medium-associated vancomycin response rewiring inFrontiers in microbiology · 2026Article
- Cold atmospheric plasma mediates antibiofilm activity and sensitization to further plasma and antibiotic challenges againstFEMS microbes · 2026Article
- D-alanine synthesis and exogenous alanine affect the antimicrobial susceptibility ofAntimicrobial agents and chemotherapy · 2025Article
- Protection Elicited by Glutamine Auxotroph ofVaccines · 2025Article
- Potential antibacterial effects and transcriptomic analysis of a novel reversible photoacid-based crystalline coordination polymer.Frontiers in microbiology · 2025Article
- Whole genome sequence and comparative genomics analysis of multidrug-resistant Staphylococcus xylosus NM36 isolated from a cow with mastitis in Basrah city.Journal, genetic engineering & biotechnology · 2023Article
- Molecular Determinants of β-Lactam Resistance in Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Review
- Role of the NaHCOMicrobiology spectrum · 2023Article
- Article
- Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria.Nature communications · 2022Article
- An Interplay of Multiple Positive and Negative Factors Governs Methicillin Resistance in Staphylococcus aureus.Microbiology and molecular biology reviews : MMBR · 2022Review
- Azithromycin resistance mutations inMicrobial genomics · 2020Article
- Inactivation of Glutamine Synthetase-Coding GeneFrontiers in microbiology · 2020Article
- Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.Scientific reports · 2018Article
- First insights of peptidoglycan amidation in Gram-positive bacteria - the high-resolution crystal structure of Staphylococcus aureus glutamine amidotransferase GatD.Scientific reports · 2018Article
- The giant protein Ebh is a determinant of Staphylococcus aureus cell size and complement resistance.Journal of bacteriology · 2014Article
- Genomic Basis for Methicillin Resistance in Staphylococcus aureus.Infection & chemotherapy · 2013Review
- Identification of genetic determinants and enzymes involved with the amidation of glutamic acid residues in the peptidoglycan of Staphylococcus aureus.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tn551 insertional inactivation of femC is known to reduce methicillin resistance levels in methicillin-resistant and -susceptible Staphylococcus aureus. By use of cotransductional crosses, femC was mapped close to thrB on the SmaI-A fragment of the S. aureus NCTC 8325 chromosome. The Tn551 insertion femC::omega 2005 was found to interrupt an open reading frame coding for a putative protein of 121 amino acids which is highly similar to the glutamine synthetase repressors (GlnR) of Bacillus spp. Downstream of femC, an open reading frame highly similar to Bacillus sp. glutamine synthetases (GlnA) was found. Northern (RNA) blots probed with putative glnR or glnA fragments revealed that 1.7- and 1.9-kb transcripts characteristic of wild-type cells were replaced by less abundant 7.0- and 7.2-kb transcripts in the femC::omega 2005 mutant. Total glutamine synthetase activity was also decreased in the mutant strain; the addition of glutamine to defined media restored the wild-type methicillin resistance phenotype of the femC mutant. This result suggests that the omega 2005 insertion in glnR has a polar effect on glnA and that glnR and glnA are transcribed together as an operon. These results suggest that the loss of wild-type levels of glutamine synthetase and the consequent decrease in glutamine availability cause a decreased level of methicillin resistance.
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