ArticleThe Journal of membrane biology1993
Single anion channels reconstituted from cardiac mitoplasts.
Article in The Journal of membrane biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Anion Channels of Mitochondria.Handbook of experimental pharmacology · 2017Review
- The Involvement of MgOxidative medicine and cellular longevity · 2017Review
- Electro-pharmacological profiles of two brain mitoplast anion channels: Inferences from single channel recording.EXCLI journal · 2017Article
- Mitochondrial chloride channels: electrophysiological characterization and pH induction of channel pore dilation.European biophysics journal : EBJ · 2013Article
- Inhibitory effect of DIDS, NPPB, and phloretin on intracellular chloride channels.Pflugers Archiv : European journal of physiology · 2007Article
- Stilbene derivatives inhibit the activity of the inner mitochondrial membrane chloride channels.Cellular & molecular biology letters · 2007Article
- Redox regulation of CLIC1 by cysteine residues associated with the putative channel pore.Biophysical journal · 2006Article
- The mitochondrial inner membrane anion channel is inhibited by DIDS.Journal of bioenergetics and biomembranes · 1996Article
- Anion channels of the inner membrane of mammalian and yeast mitochondria.Journal of bioenergetics and biomembranes · 1996Review
- A Cl channel in Ascaris suum selectivity conducts dicarboxylic anion product of glucose fermentation and suggests a role in removal of waste organic anions.The Journal of membrane biology · 1995Article
- Reconstitution of hepatic uricase in planar lipid bilayer reveals a functional organic anion channel.The Journal of membrane biology · 1995Article
- Electrophysiology of the inner mitochondrial membrane.Journal of bioenergetics and biomembranes · 1994Review
- Evidence for a K+ channel in bovine chromaffin granule membranes: single-channel properties and possible bioenergetic significance.European biophysics journal : EBJ · 1994Article
- Structural features of a multisubstate cardiac mitoplast anion channel: inferences from single channel recording.The Journal of membrane biology · 1993Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ion channels from sheep cardiac mitoplast (inverted inner mitochondrial membrane vesicle) preparations were incorporated into voltage-clamped planar lipid bilayers. The appearance of anion rather than cation channels could be promoted by exposing the bilayers to osmotic gradients formed by Cl- salts of large, relatively impermeant, cations at a pH of 8.8. Two distinct activities were identified. These comprised a multisubstate anion channel of intermediate conductance (approximately 60 pS in 300 vs. 50 mM choline Cl, approximately 100 pS in symmetric 150 mM KCl), and a lower-conductance anion channel (approximately 25 or approximately 50 pS in similar conditions), which only displayed two well-defined substates, at approximately 25 and approximately 50% of the fully open state. The larger channels were not simple multiples of the lower-conductance channels, but both discriminated poorly, and to a similar extent, between anions and cations (PCl-/Pcholine+ approximately 12, PCl-/PK+ approximately 8). The lower-conductance channel was only minimally selective between different anions (PNO3-(1.0) = PCl- > PBr- > PI- > PSCN-(0.8)), and its conductance failed to saturate even in high (> 1.0 M) activities of KCl. The channels were not obviously voltage dependent, and they were unaffected by 0.5 mM SITS, H2O2, propranolol, quinine or amitriptyline, or by 2 mM ATP, or by variations in pH (5.5-8.8). Ca2+ and Mg2+ did not alter single channel activity, but did modify single current amplitudes in the lower-conductance channel. This effect, together with voltage-dependent substate behavior, is described in the following paper.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.