ArticleImmunology1993
Ontogeny of leucocyte populations in the spleen of fetal lambs with emphasis on the early prominence of B cells.
Article in Immunology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Pulmonary Consequences of Prenatal Inflammatory Exposures: Clinical Perspective and Review of Basic Immunological Mechanisms.Frontiers in immunology · 2020Review
- Splenic development and injury in premature lambs supported by the artificial placenta.Journal of pediatric surgery · 2019Article
- Endogenous transplacental transmission of Neospora caninum during successive pregnancies across three generations of naturally infected sheep.Veterinary research · 2018Article
- Ovine fetal immune response to Cache Valley virus infection.Journal of virology · 2013Article
- Ontogeny of the immune response in the ovine lung.Immunological investigations · 2012Article
- Contributions of conventional and heavy-chain IgG to immunity in fetal, neonatal, and adult alpacas.Clinical and vaccine immunology : CVI · 2010Article
- The XC chemokine receptor 1 is a conserved selective marker of mammalian cells homologous to mouse CD8alpha+ dendritic cells.The Journal of experimental medicine · 2010Article
- Early Vlambda diversification in sheep.Immunology · 2001Article
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- Bovine leukemia virus-induced lymphocytosis and increased cell survival mainly involve the CD11b+ B-lymphocyte subset in sheep.Journal of virology · 1998Article
- Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ-free piglets: production by CD5- B cells.Immunology · 1996Article
- Fetal lambs are depleted of IgM+ cells following a single injection of an anti-IgM antibody early in gestation.Immunology · 1996Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence and distribution of B cells and other early leucocyte populations are described in the spleen of fetal lambs from 40 to 134 days of gestation (length of gestation 150 days). Computer-assisted morphometric analysis and flow cytometry were used to quantify the early predominance of B cells in mid-gestation. B cells appeared at about 48 days and increased in number to occupy over 20% of the spleen area at 77 days. All spleens were collected on their respective livers and at no stage did the livers contain more than a few IgM-positive (+) cells, which were usually close to blood vessels. Two-colour flow cytometry demonstrated that only 1-2% of IgM+ cells expressed CD5 at 81 days. Beyond 77 days, with the expanding presence of T cells, the percentage of area occupied by IgM+ cells declined to stabilize at about 7% during late gestation. The conventional organization of the splenic white pulp was observed from 90 days along with 5' nucleotidase-positive primary follicles. Double staining technique using immunohistochemical methods demonstrated that IgM+ cells were proliferating in the spleen from as early as 51 days and that clusters of proliferating IgM+ cells were prominent between 60 and 77 days. The results of the present study suggest that during the ontogeny of fetal lambs the spleen is a site of B-cell development or expansion before colonization of the ileal Peyer's patch and the subsequent generation of the preimmune antibody repertoire.
Indexed as
Identifiers
7508421PMC1422258W2416406870What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.