ArticleThe EMBO journal1994
RNA annealing activities in HeLa nuclei.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
71 citing papers in PubMed, 152 citations in OpenAlex.
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- RNA-binding proteins in disease etiology: fragile X syndrome and spinal muscular atrophy.RNA (New York, N.Y.) · 2025Review
- Evolutionary and functional insights into the Ski2-like helicase family in Archaea: a comparison of Thermococcales ASH-Ski2 and Hel308 activities.NAR genomics and bioinformatics · 2024Article
- A working model for condensate RNA-binding proteins as matchmakers for protein complex assembly.RNA (New York, N.Y.) · 2022Review
- Kinetics of protein-assisted nucleic acid interconversion monitored by transient time resolved fluorescence in microfluidic droplets.Nucleic acids research · 2021Article
- RNA matchmaking in chromatin regulation.Biochemical Society transactions · 2020Review
- Coordinate regulation of alternative pre-mRNA splicing events by the human RNA chaperone proteins hnRNPA1 and DDX5.Genes & development · 2018Article
- Slow molecular recognition by RNA.RNA (New York, N.Y.) · 2017Article
- Arginine methylation enhances the RNA chaperone activity of the West Nile virus host factor AUF1 p45.RNA (New York, N.Y.) · 2016Article
- An RNA matchmaker protein regulates the activity of the long noncoding RNA HOTAIR.RNA (New York, N.Y.) · 2016Article
- Evf2 lncRNA/BRG1/DLX1 interactions reveal RNA-dependent inhibition of chromatin remodeling.Development (Cambridge, England) · 2015Article
- A guanosine-centric mechanism for RNA chaperone function.Science (New York, N.Y.) · 2013Article
- hnRNP L and hnRNP A1 induce extended U1 snRNA interactions with an exon to repress spliceosome assembly.Molecular cell · 2013Article
- Analysis of nucleic acid chaperoning by the prion protein and its inhibition by oligonucleotides.Nucleic acids research · 2011Article
- A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA.Nucleic acids research · 2011Article
- The RNA annealing mechanism of the HIV-1 Tat peptide: conversion of the RNA into an annealing-competent conformation.Nucleic acids research · 2011Article
- Proteins with RNA chaperone activity: a world of diverse proteins with a common task-impediment of RNA misfolding.Biochemistry research international · 2011Article
- Role of RNA chaperones in virus replication.Virus research · 2009Review
- gRNA/pre-mRNA annealing and RNA chaperone activities of RBP16.RNA (New York, N.Y.) · 2008Article
- Dissecting RNA chaperone activity.RNA (New York, N.Y.) · 2007Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-RNA base pairing plays a critical role in the interactions between pre-mRNAs and trans-acting factors during the processing of pre-mRNAs (hnRNAs) into mRNAs, and it is likely that specific factors are required to promote the annealing of RNAs. To identify particular nuclear components that have such activity, we fractionated HeLa nucleoplasm and assayed for activity which promoted the hybridization of a pre-mRNA with an antisense RNA probe complementary to 60 nucleotides (nt) encompassing the 3' splice site. At least nine major RNA annealing activities were identified and, surprisingly, eight of these copurified partially or to homogeneity with known hnRNP proteins. The activities of three of these proteins, hnRNP A1, C1 and U, were confirmed using purified recombinant proteins. Moreover, we found that the RNA binding domain alone of hnRNP C1/C2 had significant activity, indicating that this RNA annealing may result, at least partly, from chaperone activity: a direct modulation of RNA conformation by hnRNP proteins. The finding that hnRNP proteins have strong RNA annealing activity indicates that they can profoundly affect the interactions of pre-mRNAs with trans-acting factors and suggests this to be an important function of hnRNP proteins in the processing of pre-mRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.