ArticleMolecular biology of the cell1993
The Gly/Arg-rich (GAR) domain of Xenopus nucleolin facilitates in vitro nucleic acid binding and in vivo nucleolar localization.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Biosensor-guided discovery of peptide inhibitors targeting the ribosomal protein uS5-PDCD2 chaperone interaction.The Journal of biological chemistry · 2026Article
- New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida).Protoplasma · 2025Article
- Emergent microenvironments of nucleoli.Nucleus (Austin, Tex.) · 2024Review
- RG/RGG repeats in the C. elegans homologs of Nucleolin and GAR1 contribute to sub-nucleolar phase separation.Nature communications · 2022Article
- HeterozygousGenes · 2021Article
- Nucleolin (NCL) inhibits the growth of peste des petits ruminants virus.The Journal of general virology · 2020Article
- Nucleolin modulates compartmentalization and dynamics of histone 2B-ECFP in the nucleolus.Nucleus (Austin, Tex.) · 2018Article
- Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58).Journal of biomedical science · 2015Article
- Identification and functional analysis of NOL7 nuclear and nucleolar localization signals.BMC cell biology · 2010Article
- RNAi knockdown of Nopp140 induces Minute-like phenotypes in Drosophila.Molecular biology of the cell · 2007Article
- Subnucleolar location of fibrillarin and NopA64 in Lepidium sativum root meristematic cells is changed in altered gravity.Protoplasma · 2006Article
- Novel checkpoint response to genotoxic stress mediated by nucleolin-replication protein a complex formation.Molecular and cellular biology · 2005Article
- A family of RS domain proteins with novel subcellular localization and trafficking.Nucleic acids research · 2005Article
- Article
- Two splice variants of Nopp140 in Drosophila melanogaster.Molecular biology of the cell · 2002Article
- The carboxy-terminal fragment of nucleolin interacts with the nucleocapsid domain of retroviral gag proteins and inhibits virion assembly.Journal of virology · 2000Article
- The classical progesterone receptor mediates Xenopus oocyte maturation through a nongenomic mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Identification of proteins that associate with protein kinase CK2.Molecular and cellular biochemistry · 1999Article
- "Micronucleoli" in the Xenopus germinal vesicle.Chromosoma · 1997Article
- Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epitope-tagged Xenopus nucleolin was expressed in Escherichia coli cells and in Xenopus oocytes either as a full-length wild-type protein or as a truncation that lacked the distinctive carboxy glycine/arginine-rich (GAR) domain. Both full-length and truncated versions of nucleolin were tagged at their amino termini with five tandem human c-myc epitopes. Whether produced in E. coli or in Xenopus, epitope-tagged full-length nucleolin bound nucleic acid probes in in vitro filter binding assays. Conversely, the E. coli-expressed GAR truncation failed to bind the nucleic acid probes, whereas the Xenopus-expressed truncation maintained slight binding activity. Indirect immunofluorescence staining showed that myc-tagged full-length nucleolin properly localized to the dense fibrillar regions within the multiple nucleoli of Xenopus oocyte nuclei. The epitope-tagged GAR truncation also translocated to the oocyte nuclei, but it failed to efficiently localize to the nucleoli. Our results show that the carboxy GAR domain must be present for nucleolin to efficiently bind nucleic acids in vitro and to associate with nucleoli in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.