ArticleInfection and immunity1994
Induction of Mycobacterium avium gene expression following phagocytosis by human macrophages.
Article in Infection and immunity, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 137 citations in OpenAlex.
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- Mycobacterium avium subsp. Paratuberculosis and the expression of selected virulence and pathogenesis genes in response to 6°C, 65°c and ph 2.0.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Development of a single-step subtraction method for eukaryotic 18S and 28S ribonucleic acids.Journal of biomedicine & biotechnology · 2011Article
- Novel attenuated Salmonella enterica serovar Choleraesuis strains as live vaccine candidates generated by signature-tagged mutagenesis.Infection and immunity · 2005Article
- Utilization of a ts-sacB selection system for the generation of a Mycobacterium avium serovar-8 specific glycopeptidolipid allelic exchange mutant.Annals of clinical microbiology and antimicrobials · 2004Article
- Identification of Porphyromonas gingivalis genes specifically expressed in human gingival epithelial cells by using differential display reverse transcription-PCR.Infection and immunity · 2004Article
- Mutational analysis of cell wall biosynthesis in Mycobacterium avium.Journal of bacteriology · 2003Article
- Mycobacterium avium genes expressed during growth in human macrophages detected by selective capture of transcribed sequences (SCOTS).Infection and immunity · 2002Article
- Identification of Escherichia coli genes that are specifically expressed in a murine model of septicemic infection.Infection and immunity · 2002Article
- Characterization of a Mycobacterium intracellulare variant strain by molecular techniques.Journal of clinical microbiology · 2001Article
- Detection of differential gene expression in biofilm-forming versus planktonic populations of Staphylococcus aureus using micro-representational-difference analysis.Applied and environmental microbiology · 2001Article
- Acid-induced gene expression in Helicobacter pylori: study in genomic scale by microarray.Infection and immunity · 2001Article
- Isolation of regulated genes of the cyanobacterium Synechocystis sp. strain PCC 6803 by differential display.Journal of bacteriology · 2000Article
- Identification of ragAB as a temperature-regulated operon of Porphyromonas gingivalis W50 using differential display of randomly primed RNA.Infection and immunity · 2000Article
- Measurement of bacterial gene expression in vivo.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Questions about the behaviour of bacterial pathogens in vivo.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- In vivo gene expression and the adaptive response: from pathogenesis to vaccines and antimicrobials.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Little is known about the bacterial factors that enable pathogenic mycobacteria to survive and multiply within the macrophages of the infected host. By preparing cDNA from Mycobacterium avium bacilli grown in human-derived macrophages and in broth culture and using subtractive hybridization to remove commonly expressed genes, a procedure was developed to identify genes of M. avium that are specifically expressed when the bacilli are growing within macrophages. Total RNA was isolated from M. avium recovered 5 days after infection of human macrophages and from bacilli grown in vitro in broth. Mycobacterial mRNAs were converted to cDNA by reverse transcription. Biotin-modified cDNAs prepared from M. avium grown in broth culture were used to subtract the housekeeping genes from the cDNAs of the macrophage-derived M. avium. After each round of subtraction, a sample of the unsubtracted cDNA was amplified, labeled, and hybridized to cosmid clones of M. avium DNA. After three rounds of subtraction, the amplified DNA hybridized to approximately 1% of the cosmid clones under stringent conditions. Although the majority of the genes that are induced in phagocytized M. avium cells are expressed in the broth-grown bacilli, one DNA fragment that was identified coded for an mRNA that is highly specific for M. avium in phagosomes. This procedure will be especially useful for identifying genes that are expressed in response to growth in specific environments from organisms with genetic systems that are not well characterized.
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