ArticleMolecular and cellular biology1994
Tyrosine phosphorylation of CD45 phosphotyrosine phosphatase by p50csk kinase creates a binding site for p56lck tyrosine kinase and activates the phosphatase.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 131 citations in OpenAlex.
- A multi-omic approach reveals utility of CD45 expression in prognosis and novel target discovery.Frontiers in genetics · 2022Article
- Protein tyrosine phosphatase receptor type C (PTPRC or CD45).Journal of clinical pathology · 2021Review
- Article
- A Phosphosite within the SH2 Domain of Lck Regulates Its Activation by CD45.Molecular cell · 2017Article
- Article
- C-terminal Src kinase-mediated EPIYA phosphorylation of Pragmin creates a feed-forward C-terminal Src kinase activation loop that promotes cell motility.Cancer science · 2016Article
- Receptor-type Protein tyrosine phosphatase β regulates met phosphorylation and function in head and neck squamous cell carcinoma.Neoplasia (New York, N.Y.) · 2012Article
- Receptor type protein tyrosine phosphatases (RPTPs) - roles in signal transduction and human disease.Journal of cell communication and signaling · 2012Article
- Article
- Extracellular domain dependence of PTPalpha transforming activity.Genes to cells : devoted to molecular & cellular mechanisms · 2010Article
- CD45, CD148, and Lyp/Pep: critical phosphatases regulating Src family kinase signaling networks in immune cells.Immunological reviews · 2009Review
- CD45: all is not yet crystal clear.Immunology · 2006Review
- Receptor protein tyrosine phosphatase from stem cells to mature glial cells of the central nervous system.Journal of molecular neuroscience : MN · 2006Review
- Integrin CD11a cytoplasmic tail interacts with the CD45 membrane-proximal protein tyrosine phosphatase domain 1.Immunology · 2005Article
- Models of signal transduction through the B-cell antigen receptor.Immunology · 2003Review
- Article
- Autonomous maturation of alpha/beta T lineage cells in the absence of COOH-terminal Src kinase (Csk).The Journal of experimental medicine · 2001Article
- Comparative study of protein tyrosine phosphatase-epsilon isoforms: membrane localization confers specificity in cellular signalling.The Biochemical journal · 2001Article
- A phosphotyrosine displacement mechanism for activation of Src by PTPalpha.The EMBO journal · 2000Article
- Differential effects of B cell receptor and B cell receptor-FcgammaRIIB1 engagement on docking of Csk to GTPase-activating protein (GAP)-associated p62.The Journal of experimental medicine · 1997Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Src family protein tyrosine kinases (PTKs) play an essential role in antigen receptor-initiated lymphocyte activation. Their activity is largely regulated by a negative regulatory tyrosine which is a substrate for the activating action of the CD45 phosphotyrosine phosphatase (PTPase) or, conversely, the suppressing action of the cytosolic p50csk PTK. Here we report that CD45 was phosphorylated by p50csk on two tyrosine residues, one of them identified as Tyr-1193. This residue was not phosphorylated by T-cell PTKs p56lck and p59fyn. Tyr-1193 was phosphorylated in intact T cells, and phosphorylation increased upon treatment with PTPase inhibitors, indicating that this tyrosine is a target for a constitutively active PTK. Cotransfection of CD45 and csk into COS-1 cells caused tyrosine phosphorylation of CD45 in the intact cells. Tyrosine-phosphorylated CD45 bound p56lck through the SH2 domain of the kinase. Finally, p50csk-mediated phosphorylation of CD45 caused a severalfold increase in its PTPase activity. Our results show that direct tyrosine phosphorylation of CD45 can affect its activity and association with Src family PTKs and that this phosphorylation could be mediated by p50csk. If this is also true in the intact cells, it adds a new dimension to the physiological function of p50csk in T lymphocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.