ArticleThe American journal of pathology1993
Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis.
Article in The American journal of pathology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 128 citations in OpenAlex.
- Small airway lesions appear with the course of IPF and relate to the severity of pulmonary fibrosis progression.BMC pulmonary medicine · 2025Observational
- Cromolyn sodium and masitinib combination inhibits fibroblast-myofibroblast transition and exerts additive cell-protective and antioxidant effects on a bleomycin-induced in vitro fibrosis model.Pharmacology research & perspectives · 2024Article
- The Plastic Interplay between Lung Regeneration Phenomena and Fibrotic Evolution: Current Challenges and Novel Therapeutic Perspectives.International journal of molecular sciences · 2023Review
- Fibroblasts-Warriors at the Intersection of Wound Healing and Disrepair.Biomolecules · 2023Review
- The Regenerative Power of Stem Cells: Treating Bleomycin-Induced Lung Fibrosis.Stem cells and cloning : advances and applications · 2023Review
- Review
- The Role of Rho GTPases During Fibroblast Spreading, Migration, and Myofibroblast Differentiation in 3D Synthetic Fibrous Matrices.Cellular and molecular bioengineering · 2021Article
- The contribution of animal models to understanding the role of the immune system in human idiopathic pulmonary fibrosis.Clinical & translational immunology · 2020Article
- Distal airway stem cells ameliorate bleomycin-induced pulmonary fibrosis in mice.Stem cell research & therapy · 2019Article
- The Tcf21 lineage constitutes the lung lipofibroblast population.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- β-Catenin/Smad3 Interaction Regulates Transforming Growth Factor-β-Induced Epithelial to Mesenchymal Transition in the Lens.International journal of molecular sciences · 2019Article
- Attenuation of lipopolysaccharide-induced lung inflammation by ascorbic acid in rats: Histopathological and ultrastructural study.SAGE open medicine · 2019Article
- Effect of Chamomile Hydroalcoholic Extract on Bleomycin-Induced Pulmonary Fibrosis in Rat.Tanaffos · 2018Article
- Deletion of SMARCA4 impairs alveolar epithelial type II cells proliferation and aggravates pulmonary fibrosis in mice.Genes & diseases · 2017Article
- Myofibroblasts and lung fibrosis induced by carbon nanotube exposure.Particle and fibre toxicology · 2016Review
- Lung Pericytes and Resident Fibroblasts: Busy Multitaskers.The American journal of pathology · 2016Review
- EDA Fibronectin in Keloids Create a Vicious Cycle of Fibrotic Tumor Formation.The Journal of investigative dermatology · 2015Article
- Article
- Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats.Experimental and therapeutic medicine · 2012Article
- Caveolin-1 signaling in lung fibrosis.The open rheumatology journal · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of fibroblasts in alveolar septa are characterized by the presence of cytoplasmic bundles of microfilaments that contain cytoplasmic actin isoforms; these cells have been named contractile interstitial cells or V-type myofibroblasts. In the rat, they express desmin as intermediate filament protein. In this study, we explored the possibility that modulation and replication of such septal fibroblasts result in the appearance of alpha-smooth muscle (alpha-SM) actin-positive myofibroblasts, typical of lung fibrosis. Experimental pulmonary fibrosis was produced by a unique intratracheal instillation of bleomycin to 28 rats. Eight additional rats used as controls received the equivalent volume of saline. Paraffin and frozen sections of lungs were examined at days 1, 3, 5 and 7 after treatment. Microfilaments and intermediate filaments were stained using antibodies against total actin, alpha-SM actin, desmin, vimentin, keratin, and SM myosin. Electron microscopic labeling of desmin and alpha-SM actin using immunogold technique was done on Lowicryl K4M resin-embedded specimens. alpha-SM actin appeared in desmin-positive alveolar fibroblasts as early as 24 hours after intratracheal bleomycin instillation; the modulation of alpha-SM actin in these cells was preceded by a lymphomonocytic infiltration of alveolar septa. Twenty-four hours to 3 days after bleomycin administration, a proliferation of alveolar myofibroblasts occurred. Fibrosis with laying down of collagen fibers took place after the above mentioned cellular modifications. Our results support the view that septal fibroblastic cells can modulate into typical alpha-SM actin-containing myofibroblasts during experimental bleomycin-induced pulmonary fibrosis. In such a modulation a possible role of cytokines, particularly of transforming growth factor-beta, is considered.
Indexed as
Identifiers
7504890PMC1887256W1507642266What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.