ArticleProceedings of the National Academy of Sciences of the United States of America1993
A conserved helix motif complements the protein kinase core.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Crustacean Protein Kinases A and C: Bioinformatic Characterization in Decapods and Other Non-Model Organisms.International journal of molecular sciences · 2025Article
- Article
- The Tails of Protein Kinase A.Molecular pharmacology · 2022Review
- A tripartite cooperative mechanism confers resistance of the protein kinase A catalytic subunit to dephosphorylation.The Journal of biological chemistry · 2020Article
- Cleavage Alters the Molecular Determinants of Protein Kinase C-δ Catalytic Activity.Molecular and cellular biology · 2017Article
- Conformational equilibrium of N-myristoylated cAMP-dependent protein kinase A by molecular dynamics simulations.Biochemistry · 2012Article
- Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study.BMC structural biology · 2009Article
- Structural basis of protein kinase C isoform function.Physiological reviews · 2008Review
- Signalling specificity of Ser/Thr protein kinases through docking-site-mediated interactions.The Biochemical journal · 2003Review
- FRET-based detection of different conformations of MK2.EMBO reports · 2001Article
- Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation.The Journal of cell biology · 2000Article
- Cell specific transformation by c-fms activating loop mutations is attributable to constitutive receptor degradation.Oncogene · 1999Article
- Organization and alternative splicing of the Caenorhabditis elegans cAMP-dependent protein kinase catalytic-subunit gene (kin-1).The Biochemical journal · 1999Article
- Conserved water molecules contribute to the extensive network of interactions at the active site of protein kinase A.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Importance of the A-helix of the catalytic subunit of cAMP-dependent protein kinase for stability and for orienting subdomains at the cleft interface.Protein science : a publication of the Protein Society · 1997Article
- Src homology domains of v-Src stabilize an active conformation of the tyrosine kinase catalytic domain.Molecular and cellular biochemistry · 1996Article
- Functional domains of fused, a serine-threonine kinase required for signaling in Drosophila.Genetics · 1996Article
- Dual role of cAMP during Dictyostelium development.Experientia · 1995Review
- Molecular cloning and characterization of a conserved nuclear serine(threonine) protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Mutagenic structure/function analysis of the cytoplasmic cysteines of the insulin receptor.The Biochemical journal · 1995Article
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5 authors.
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Abstract
Residues 40-300 of the mammalian catalytic (C) subunit of cAMP-dependent protein kinase define a conserved bilobal catalytic core shared by all eukaryotic protein kinases. Contiguous to the core is an extended amphipathic alpha-helix (A helix). Trp30, a prominent feature of this helix, fills a deep hydrophobic pocket between the two lobes on the surface opposite to the active site. The C subunit in Dictyostelium discoideum shows sequence conservation of residues 40-350 with the mouse enzyme but contains an N-terminal extension of 332 residues. A sequence corresponding to the A helix contiguous to the core is absent. However, we have now identified a remote A-helix motif (residues 77-98). When the core of the Dictyostelium C subunit was modeled, based on the mouse C subunit, complementarity between this putative A helix and the surface of the core was found to be conserved. Analysis of other protein kinases reveals that the A-helix motif is not restricted to cAMP-dependent protein kinase. In the Src-related family of protein kinases, for example, an A helix is very likely contiguous to the core, thus serving as a linker between the conserved catalytic core and the Src homology 2 domain. We predict that an A-helix motif complementary to the core will be a conserved feature of most eukaryotic protein kinases.
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